两种快速分离检测南方水牛乳乳清蛋白方法的比较  

Contrast between two rapid separation and detection of Southern Water Buffalo whey protein methods

作　　者：成希飞[1] 李昀锴[1] 向明霞[1] 徐明芳[1] 

机构地区：[1]广州暨南大学生命科学与技术学院生物工程系,广州510632

出　　处：《中国乳品工业》2012年第5期55-57,64,共4页China Dairy Industry

基　　金：广东省科技计划项目(No:2009B011300003);两种快速分离检测南方水牛乳乳清蛋白方法的比较

摘　　要：采用了毛细管电泳法(CE)和反相高效液相色谱法(RP-HPLC)两种方法对乳源乳清蛋白进行分离检测。结果表明:两种方法都能够有效分离乳清蛋白中的BSA,α-La,β-Lg,并能对各种蛋白进行定性定量分析。相比而言,毛细管电泳法分离更加快速,整个分离过程7 min左右即可完成,能分离出IgG组分。而RP-HPLC分离时间更长,需要45 min才能完全分离,并且无法分离出IgG组分。Two methods were studied for the separation and detection of whey protein by reverse-phase HPLC （RP-HPLC） and capillary electrophoresis （CE）. The results show that two methods can both separate BSA,α-La,β-Lg effectively, and both can do qualitative and quantitative analysis for different kinds of whey protein. In contrast, CE can separate more rapidly, it only used 7 minutes to complete the whole separation process while RP-HPLC needed 45 minutes, in addition, RP-HPLC cannot separate and examine the IgG component.

关 键 词：毛细管电泳法 RP-HPLC 牛奶 乳清蛋白 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]