急性脑缺血再灌注大鼠海马CA1区细胞凋亡与β-catenin、GSK-3β蛋白表达的关系  

Relationship between the expression of cell apoptosis and β-catenin and GSK-3β protein in the hippocampal CA1 of rats with acute cerebral ischemia reperfusion injury

作　　者：刘斌[1] 董晓柳[1] 张文彦[1] 张晋霞[1] 

机构地区：[1]河北联合大学附属医院,河北唐山063000

出　　处：《山东医药》2012年第17期28-31,共4页Shandong Medical Journal

基　　金：河北省医学科学研究重点课题(20110531)

摘　　要：目的探讨急性脑缺血再灌注(IR)大鼠海马CA1区细胞凋亡与Wnt信号通路β-连环蛋白(β-catenin)、糖原合成酶激酶3β(GSK-3β)蛋白表达的关系。方法选择雄性SD大鼠36只,随机分为假手术组6只、脑IR组30只,脑IR组又分为IR后3、6、24、48、72 h各6只,采用线栓法制作大鼠大脑中动脉缺血再灌注模型。应用原位末端标记法检测各组细胞凋亡情况,免疫组化法及Western blotting蛋白印记法分别检测β-catenin、GSK-3β蛋白表达;并对IR组的β-catenin、GSK-3β蛋白表达与细胞凋亡进行相关性分析。结果与假手术组比较,IR组各时间点细胞凋亡数、GSK-3β蛋白阳性数及β-catenin蛋白表达量明显增加(P均<0.01),β-catenin蛋白阳性数、GSK-3β蛋白表达量增加,但无统计学意义(P均>0.05)。脑IR后不同时间点的细胞凋亡数与GSK-3β蛋白阳性数呈正相关(P<0.05),与β-catenin蛋白阳性数无相关性(P>0.05)。结论 Wnt信号通路在脑IR组损伤中可能发挥重要作用,GSK-3β蛋白对缺血性脑损伤的细胞凋亡起促进作用。Objective To explore the relationship between the expressions of cell apoptosis and β-catenin and GSK- 3β protein in the hippocampal CA1 of rats with acute cerebral ischemia reperfusion injury. Methods A total of 36 male SD rats were divided into sham operation group （6 cases） and ischemia reperfusion （IR） group （30 cases） , IR group were also divided into 3, 6, 24, 48, 72 h subgroup after IR. Middle cerebral artery occlusion （MCAO） and reperfushion model were made by an intraluminal filament method. Cell apoptosis was deteced by TUNEL method, the expressions of β-catenin and GSK-3β protein were observed by immunohistochemistry and Western blotting protein imprinting-based respectively. Results Compared with sham operation group, the numbers of apoptotic cells at each time point were obviously increased in IR group （ all P 〈 0.01 ）, the expressions of GSK-3β protein were obviously increased （ P 〈 0.01 ）, the expressions of β-catenin protein were increased, but showing no significant difference （ P 〉 0.05 ）. The expressions of GSK-3β protein were positively correlated with the numbers of apoptotic neurons in the hippocampal CA1 of rats （ P 〈 0.05 ） , but the ex- pressions of β-catenin protein was no correlated with the numbers of apoptotic neurons （ P 〉 0.05 ）. Conclusion Wnt sig- naling pathway may play an important role in cerebral ischemia reperfusion injury, and GSK-3β protein may promote apop- tosis.

关 键 词：脑缺血再灌注损伤 细胞凋亡 Β-连环蛋白 糖原合成激酶3β蛋白 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]