茶色素体外抗氧化作用研究  

Antioxidation of Tea Pigment in Vitro

作　　者：富丽[1] 韩国柱[1] 李楠[2] 林原[1] 唐泽耀[1] 

机构地区：[1]大连医科大学药理教研室,116044 [2]大连理工大学化工学院化学教研室,116012

出　　处：《医药导报》2012年第5期562-564,共3页Herald of Medicine

基　　金：国家自然科学基金资助项目(30772601)

摘　　要：目的研究茶色素(TP)体外抗氧化作用。方法通过邻苯三酚自氧化和抗体外氢氧根法分别测定TP与阳性对照药维生素C的抗氧化作用,比较两者作用程度。结果 0.1%,0.2%,0.3%TP对邻苯三酚自氧化平均抑制率分别为每分钟7.022%,13.427%,16.910%;0.1%,0.2%维生素C分别为16.340%,32.850%。TP与维生素C对邻苯三酚自氧化平均抑制率呈剂量依赖式增加。0.1%,0.3%,0.5%,0.7%,1.0%TP对体外羟自由基(.OH)生成的平均抑制率分别为(11.00±0.70)%,(20.68±0.60)%,(27.04±0.07)%,(32.70±0.30)%,(42.90±0.70)%;0.1%,0.3%,0.5%,0.7%,1.0%维生素C对体外.OH生成的平均抑制率分别为(4.50±0.30)%,(7.40±1.00)%,(11.42±0.23)%,(14.80±0.40)%,(16.80±0.60)%。TP和维生素C对体外.OH生成抑制率均呈剂量依赖性增加。TP和维生素C对体外.OH生成均有一定抑制作用,TP抑制作用较维生素C显著。相同剂量维生素C和TP抑制作用差异有统计学意义(P<0.01);不同剂量TP对体外.OH生成的抑制作用差异有统计学意义(P<0.05)。结论 TP体外具有抗氧化作用,尤其是在体外抗氢氧根作用方面。Objective To study the antioxidation of tea pigment（TP） in vitro.Methods The antioxidation and comparation of TP with VitC as positive control were tested by auto-oxidation of pyrogallic acid and anti-hydroxide radical assay in vitro.Results The average inhibition rate per minute of TP at the dose of 0.1%,0.2%,0.3% was 7.022%,13.427%,16.910%,respectively.Whereas,the average inhibition rate per minute of VitC at 0.1%,0.2% was 16.340%,32.850%,respectively.Both TP and VitC inhibited autoxidation of pyrogallol in a dose dependent manner.Moreover,the average suppression rate of TP at 0.1%,0.3%,0.5%,0.7%,1.0% on hydroxy radical（·OH） produced in vitro was（11.00±0.70）%,（20.68±0.60）%,（27.04±0.07）%,（32.70±0.30）%（42.90±0.70）%,respectively,while it was（4.50±0.30）%,（7.40±1.00）%,（11.42±0.23）%,（14.80±0.40）%,（16.80±0.60）% for VitC at different doses,respectively.TP and VitC hindered the generation of hydroxy radical（·OH） dose dependently,especially for TP.There was significant difference between VitC and TP at the same dose（P0.01）,and betweeen TP at different doses（P0.05）.Conclusion TP has an antioxidant effect in vitro,especially on hydroxyl elimination.

关 键 词：茶色素 自氧化 FENTON反应 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]