姜黄素衍生物抗大鼠肝纤维化的作用及其机制  

Effects and its mechanisms of curcumin derivatives on experimental hepatic fibrosis in rats

作　　者：沈能[1] 邓燕红[1] 凌宁[2] 胡鹏[2] 任红[2] 彭明利[1] 

机构地区：[1]重庆医科大学病毒性肝炎研究所,重庆渝中400010 [2]重庆医科大学附属第二医院感染科,重庆渝中400010

出　　处：《中国临床药理学杂志》2012年第5期358-360,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(30771921)

摘　　要：目的观察姜黄素衍生物(Curc-OEG)抗肝纤维化作用及其机制。方法将雄性SD大鼠共分成4组:正常组、模型组、姜黄素衍生物组和姜黄素组。用四氯化碳诱导大鼠形成肝纤维化模型,2个治疗组分别尾静脉注射100 mg·kg-1Curc-OEG和灌胃400 mg·kg-1姜黄素。10周后,测肝功及肝纤维化指标、肝组织羟脯氨酸(Hpy)水平;HE、天狼星红染色,免疫组化法观察TGF-β1及α-SMA表达;RT-PCR法测TGF-β1 mRNA水平;Western blot测TGF-β1及α-SMA蛋白表达。结果与模型组比较,Curc-OEG能改善肝功及降低肝纤维化指标,降低Hpy含量及肝纤维化程度,抑制TGF-β1及α-SMA的表达。结论 Curc-OEG具有明显的抗肝纤维化作用。Objective To investigate the effects and its mechanisms of curcumin derivatives（Curc-OEG） on experimental rat hepatic fibrosis.MethodsMale SD rats were divided into four groups ：normal group,model group,curcumin derivatives group and curcumin group.Rat model of hepatic fibrosis was induced by CCl4,and then the model rats given with intravenous injection of 100 mg·kg^-1 Curc-OEG and 400 mg·kg^-1 curcumin.After treatment for 10 weeks,liver function,serum markers of hepatic fibrosis,liver Hpy content level were detected.The HE and Sirius red staining of hepatic fibrosis were observed and the progression of hepatic fibrosis was to grade,immunohistochemical for TGF-β1 and α-SMA in rat liver tissue were distributed.The liver tissue of TGF-β1 and Collagen I mRNA levels were detected by RT-PCR.The protein expression of TGF-β1 and α-SMA were detected by Western blot.ResultsCompared with model group,Curc-OEG could improve liver function and reduce serum levels of liver fibrosis,decrease levels of liver Hpy content,inhibitting the expression of cytokine TGF-β1 and α-SMA in liver tissue.ConclusionThe Curc-OEG has a significant effect of anti-liver fibrosis.

关 键 词：肝纤维化 姜黄素衍生物 姜黄素 转化生长因子Β1 平滑肌肌动蛋白α 

分 类 号：R575.5[医药卫生—消化系统] R285.5[医药卫生—内科学]