高效液相色谱-串联质谱法测定食品中硝磺草酮  

Determination of Mesotrione in Food by Liquid Chromatography Tandem Mass Spectrometry

作　　者：张代辉[1,2] 滕国生[3] 李正强[1] 李爱军[2] 康明芹[2] 牟峻[2] 

机构地区：[1]吉林大学生命科学学院,长春130012 [2]吉林出入境检验检疫局,长春130062 [3]长春工业大学,长春130012

出　　处：《分析化学》2012年第5期811-812,共2页Chinese Journal of Analytical Chemistry

基　　金：国家质检总局行业标准基金(No.2009B160)资助项目

摘　　要：1引言 硝磺草酮（Mesotrione）,化学名2-（4-甲磺酰基-2-硝基苯甲酰基）环己烷-1,3-二酮,又名甲基磺草酮、硝磺酮,是瑞士先正达公司发明的玉米田芽前和苗后广谱选择性除草剂,因具有低毒性、高活性、对环境友好等特点[1,2],是近几年广泛使用的除草剂。但最近研究发现,硝磺草酮具有高效的起始活性和残留活性,A method for the determination of mesotrione in foods by liquid chromatography tandem mass spectrometry（LC-MS/MS） has been developed.The mesotrione in food samples was extracted with acetonitrile-water（3∶1,V/V）.The extract was cleaned up by gel permeation chromatography（GPC） and solid phase extraction cartridges（SPE）,and then diluted to a certain volume with methanol.The mobile phase in LC was an acetonitrile-0.1% formic acid aqueous solution.Electrospray ionization（ESI） source in negative ionization mode as well as multiple reaction monitoring（MRM） mode was used in the detection.The final solution was determined and confirmed by LC-MS/MS,and quantified by the external standard method.While the fortified levels of mesotrione were in the range of 0.01-0.10 mg/kg,the recoveries were 85.5%-98.1%,and the RSDs were in the range of 3.0%-6.1%.The limit of determination was 0.01 mg/kg.The method is highly sensitive and well repeatable for the determination of mesotrione in foods.

关 键 词：Liquid chromatography-tandem mass SPECTROMETRY Food MESOTRIONE 

分 类 号：TS207.53[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]