纤维素酶催化与三液相萃取偶联制备盾叶薯蓣皂苷元  

Preparation of diosgenin by integrated bioprocess of cellulase catalysis and extration in three-liquid-phase system

作　　者：魏夺[1] 董悦生[1] 韩松[1] 修志龙[1] 

机构地区：[1]大连理工大学生命科学与技术学院,辽宁大连116024

出　　处：《化工学报》2012年第6期1877-1882,共6页CIESC Journal

基　　金：国家重点基础研究发展计划项目(2009CB724706)~~

摘　　要：引言薯蓣皂苷元被誉为＂药用黄金＂、＂激素之母＂,是生产甾体激素类药物的重要原料[1-2]。盾叶薯蓣中甾体皂苷含量丰富,主要有盾叶新苷、三角叶皂苷、葡萄糖三糖苷、葡萄糖二糖苷、Using coupling biotech of cellulase-catalysis and extrusion in three-liquid-phase system that composed of n-hexane,1,4-dioxane and ammonium sulfate to hydrolyze dioscin in Chinese yan and glucose connected to steroidal saponins,and extration separation to collect diosgenin.The distribution of several steroidal saponins,hydrolyzed glucose,enzyme and its activity in the three-phase system were studied,and catalytic conversion by cellulase-enzyme and formation of diosgenin in three-liquid,organic and aqueous phases were compared.The results showed that diosgenin yield in the three-liquid-phase system could compare with that in organic phase and aqueous phase.The three-liquid-phase system could be suitable for simultaneous enzymatic hydrolysis of steroidal saponins and extration of diosgenin and glucose.The substrate and enzyme were held in the middle phase of 1,4-dioxane,whiles the products,diosgenin and glucose,were partitioned into the top and bottom phase,respectively.The yields of diosgenin was 69.4% at 96 h in the three-liquid-phase system,which was twice and 27.6 folds obtained in organic phase and aqueous phase respectively.

关 键 词：薯蓣皂苷元 三液相萃取 酶催化转化 纤维素酶 

分 类 号：TQ033[化学工程]