p16^(INK4a)、FGF-2和D2-40与宫颈癌浸润转移的关系  

The relationships between p16^(INK4a),FGF-2 a,D2-40 expression and invasion or metastasis of cervical carcinoma

作　　者：姬文莉[1] 李文婷[1] 叶明[1] 房新志[1] 

机构地区：[1]新疆医科大学附属肿瘤医院病理科,乌鲁木齐830011

出　　处：《新疆医科大学学报》2012年第5期608-612,共5页Journal of Xinjiang Medical University

摘　　要：目的探讨肿瘤抑制基因p16INK4a、成纤维细胞生长因子-2(FGF-2)和D2-40在CIN3、微小浸润癌、宫颈浸润癌中的表达及其在宫颈癌浸润转移中的作用。方法采用免疫组化学方法,检测35例CIN3、13例微小浸润癌及62例宫颈浸润癌中p16INK4a、FGF-2和D2-40蛋白的表达情况。结果 p16INK4a在微小浸润癌中的过表达率明显高于CIN3(P<0.01);FGF-2在CIN3、微小浸润癌和宫颈浸润鳞癌中的阳性率分别为51.43%、53.85%、76.92%,差异有统计学意义(P<0.05);FGF-2的表达强度与宫颈病变的发展程度呈正相关(P<0.01)。p16INK4a和FGF-2的阳性强度与宫颈癌淋巴结转移呈正相关(P<0.01或P<0.05),FGF-2与宫颈癌浸润深度有关,二者均与宫颈癌的肿瘤大小、组织学分级、临床分期无相关性;D2-40的阳性反应与临床病理因素无相关性。p16INK4a、FGF-2的表达和D2-40阳性反应之间无相关性(P>0.05)。结论 p16INK4a异常表达可能有助于诊断微小浸润癌,其阳性强度与宫颈癌淋巴结转移正相关;检测FGF-2在宫颈活检组织中的表达,对评估宫颈病变的发展以及宫颈癌浸润和转移的情况有重要的临床参考价值。Objective To investigate the expression of the p16^INK4a, the fibroblast growth factor 2 （FGF-2）, D2-40 in CIN3, microinvasive carcinoma and invasive carcinoma of cervix, and to examine the relationship between the expression of them and the cervical carcinoma invasion or lymph node metastasis. Met-hods Immunohistochemistry method was used to examine the expressions of p16INK4a , FGF-2 and D2-40 in 35 cases of CIN3,13 cases of microinvasive carcinoma and 62 cases of invasive carcinoma of cervix. Res-ults Over expression of the p16^INK4a protein was significantly higher in microinvasive carcinoma than that in CIN3 （P〈0.01）. The positive rates of FGF-2 in CIN3, microinvasive carcinoma and invasive spuamous cell carcinoma of cervix were 51. 43%, 53. 85%, 76. 92%, respectively （P〈0.05）; The expression strength of FGF-2 was correlated significantly with the extent and development of cervical lesion （P〈0.01）. The positive strength ofp16^INK4a and FGF-2 was significant correlation with lymph node metastasis in cervical carcinoma （P〈0.01 or P〈0.05）, the expression of FGF-2 was correlated significantly with depth of stroma infiltration, while no significant association was found with tumor size, histological types and clinical stage. The expression of D2-40 was no correlation with any clinical characteristic. The expres-sion of p16^INK4a, FGF-2 and D2-40 was no significant association compared with each group （P〉0.05）. Conclusion Abnormal expression of p16^INK4a may be helpful to the diagnosis of microinvasive carcinoma, the positive strength of p16^INK4a was significant correlated with lymph node metastasis; To examine the ex-pression of FGF-2 in cervical biopsy tissue may be taken as an important clinical reference value for the e- valuation of the development of cervical lesions, and the invasion and metastasis of cervical carcinoma.

关 键 词：宫颈肿瘤 转移 P16INK4A FGF-2 D2-40 

分 类 号：R737.33[医药卫生—肿瘤]