莪术油对胃癌细胞SGC-7901增殖和凋亡的影响  

The effect of proliferation and apoptosis in gastric cancer cell line SGC-7901 induced by Zedoary turmeric oil

作　　者：孙宏岩[1] 邵淑丽[1] 李爽[1] 张伟伟[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《齐齐哈尔大学学报（自然科学版）》2012年第4期33-37,共5页Journal of Qiqihar University(Natural Science Edition)

基　　金：黑龙江省自然科学基金项目(C200624);黑龙江省教育厅科学技术项目(11511447;12511611)

摘　　要：研究莪术油影响人胃癌细胞SGC-7901增殖及凋亡的机制。采用台盼蓝拒染法检测不同剂量莪术油对胃癌细胞SGC-7901的生长抑制率;光学显微镜观察细胞的形态学变化;琼脂糖凝胶电泳检测细胞DNA片段化情况;流式细胞术检测细胞线粒体膜电位的改变、细胞凋亡率以及细胞周期分布。实验结果表明:作用48h时,最佳浓度为110μg/mL,IC50值为104.958μg/mL。DNA电泳可见有梯状条带出现,流式细胞术法显示有凋亡峰出现。莪术油可诱导胃癌SGC-7901细胞凋亡。To study the effect of Zedoary turmeric oil on proliferation and apoptosis of human gastric cancer cell line SGC-7901.The growth inhibition rate of different concentration of Zedoary turmeric oil on human gastric cancer cell line SGC-7901 were detected by trypan blue stain assay. Optical microscopy observation of cell morphological changes. DNA fragmentation detected by agarose gel electrophoresis. Flow cytometry was used to detect the changes of cell mitoehondrial membrane potential, apoptosis rate and cell cycle distribution. The results shows that the optimal concentration was 110μg/mL for 48 h, and the IC50 values were 104.958μg/mL. The typical DNA ladder on agamse gel electrophoresis, the apoptotic peak was showed by FCA detection. Conclusion： the Zedoary turmeric oil can induce apoptosis of gastric cancer cell SGC-7901.

关 键 词：莪术油 胃癌 SGC-7901 细胞凋亡 

分 类 号：R73-362[医药卫生—肿瘤]