异源β-Casein基因启动子调控下的t-PAcDNA在兔乳腺中的暂性表达  

TEMPORARY EXPRESSION OF t\|PA cDNA IN RABBIT MAMMARY GLAND DRIVEN BY HETEROGENOUS β\|CASEIN PROMOTER

作　　者：李银聚[1] 侯玉泽[1] 赖良学 赵君[2,3] 金宁一 殷震[2,3] 王占彬 康怀彬[1] 

机构地区：[1]洛阳农业高等专科学校 [2]1.洛阳农业高等专科学校 [3]2解放军农牧大学,吉林长春130062

出　　处：《江苏农业研究》2000年第1期33-36,共4页

摘　　要：β-酪蛋白 (β-Casein)是哺乳动物乳汁中主要的蛋白质 ,其基因 5′侧翼调控区常作为乳腺定位表达的调控序列。表达载体 p SVL-βr-PA和 p SVL-βb-PA分别含有 1 .0 kb的大鼠β-Casein和 0 .6kg的牛β-Casein基因调控序列及 t-PAc DNA。采用基因直接注射方法将两种表达载体分别注入家兔乳腺中。溶圈试验结果显示 ,两种表达载体均能在兔乳腺细胞中得到表达 。Two mammary gland\|specific expression vectors were constructed with 1.0 kb (rat) or 0.6 kb (bovine) 5′ regulatory region of β\|casein gene and cDNA encoding human tissue\|type plasminogen activator (t\|PA). The vectors mixed with liposome were injected into the mammary glands of pregnant rabbits through the mammary gland duct and the rabbits were milked at day 1, 3, 5, 8 and 10 after parturition. Fibrin clot lysis assay showed that the 5′ regulatory regions of β\|casein gene from the two species were able to direct low level expression of t\|PA cDNA.

关 键 词：Β-酪蛋白基因 调控序列 乳腺 兔 T-PA 定位表达 

分 类 号：R394.8[医药卫生—医学遗传学] TQ464.8[医药卫生—基础医学]