血清学指标在兔肝纤维化模型中的实验研究  

Experimental study on serum fibrosis biomarkers in hepatic fibrosis rabbit model

作　　者：彭婕[1] 冯菲[1] 潘桂霞[1] 彭烨[1] 李晓栋[1] 张桉瑜[1] 董爱生[1] 左长京[1] 

机构地区：[1]第二军医大学附属长海医院核医学科,上海200433

出　　处：《山西医科大学学报》2012年第5期321-324,397,共5页Journal of Shanxi Medical University

基　　金：国家自然科学基金青年科学基金资助项目(81000601)

摘　　要：目的探讨肝纤维化血清学指标(HA、PCⅢ、LN及Ⅳ-C)与肝纤维化程度的关系。方法 45只实验兔随机分为实验组(n=36)和正常对照组(n=9)。实验组腹腔注射四氯化碳(CCl4)制作肝纤维化模型,于首次注射后第6,9,12周分批行肝纤维化血清指标检查,同时取兔肝脏行病理检查,并按病理结果分期、分级。结果共30只实验兔完成检测,其中正常肝脏9例,轻度肝纤维化6例,中度肝纤维化7例,重度肝纤维化8例。随着肝纤维化程度加重,HA、PCⅢ、LN及Ⅳ-C均逐渐增高,与肝纤维化分期之间存在正相关关系(P<0.05),其中以Ⅳ-C与纤维化分期之间关系最密切(r=0.718,P<0.05)。结论血清学指标可以反映肝纤维化的严重程度,其中以Ⅳ-C的价值较高。Objective To explore the relationship between serum fibrosis biomarkers and stages of hepatic fibrosis. Methods Forty-five rabbits were randomly divided into experiment group（n=36） and control group（n=9）.Hepatic fibrosis models were established by intraperitoneal injection of carbon tetrachloride（CCl4） in experiment rabbits.Serum fibrosis biomarkers（hyaluronic acid,type Ⅲ procollagen,laminin,type Ⅳ collagen） and pathology were observed respectively at the 6th,9th and 12th week after the first injection.According to pathological grade and stage,the rabbits were grouped. Results Thirty rabbits completed the detection,including 9 cases of normal liver,6 cases of mild liver fibrosis,7 cases of moderate liver fibrosis and 8 cases of severe liver fibrosis.With the aggravation of hepatic fibrosis,hyaluronic acid,type Ⅲ procollagen,laminin,type Ⅳ collagen were increased gradually.All those four biomarkers were positively correlated with histopathological staging（P0.05）,especially type Ⅳ collagen（r=0.718,P0.05）. Conclusion Serum fibrosis biomarkers are effective indices to evaluate the severity of hepatic fibrosis.Ⅳ-C is the best for the diagnosis of hepatic fibrosis in the four biomarkers.

关 键 词：肝纤维化 兔 动物模型 血清标志物 

分 类 号：R575[医药卫生—消化系统]