不完全性脑缺血后大鼠认知功能的变化及其病理学机制的研究  

Changes of postoperative cognitive function after incomplete cerebral ischemia in rats and its pathological mechanism

作　　者：李伟[1] 滕云鹏[1] 薛荣亮[1] 张瑛[1] 

机构地区：[1]西安交通大学医学院第二附属医院麻醉科,西安710004

出　　处：《山西医科大学学报》2012年第5期327-331,共5页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(81071071)

摘　　要：目的观察不完全性脑缺血后大鼠认知功能的变化和脑海马神经元的凋亡。方法①雄性SD大鼠30只,随机分成两组(n=15):假手术组(SO组)和缺血组,采用双侧颈总动脉夹闭法建立大鼠不完全性脑缺血模型,SO组仅解剖暴露双侧颈总动脉而不予夹闭。分别于缺血后2,3,4,5,7 d应用Morris水迷宫进行认知功能检测。②雄性SD大鼠72只,随机分为假手术组和缺血组(各36只),每组又根据缺血后时间的不同分为6组,每组6只,两组大鼠分别于缺血后24 h、48 h、72 h、4 d、5d和7 d处死大鼠,断头取脑,分离提取海马组织,流式细胞仪检测海马神经元凋亡率。结果 Morris水迷宫定位航行实验显示,与SO组相比,缺血组大鼠在训练的第1天逃避潜伏期延长,但差异没有统计学意义(P>0.05),第2,3天缺血组大鼠逃避潜伏期相比SO组明显延长(P<0.05)。空间搜索实验显示:缺血组大鼠经过虚拟平台次数和虚拟平台停留时间均少于SO组大鼠(P<0.05)。流式细胞仪结果显示:SO组大鼠随时间的延长,凋亡率没有明显变化;缺血组大鼠缺血后24 h凋亡率开始升高,至72 h达到高峰,以后逐渐下降。与SO组相比,缺血组各时间点凋亡率明显升高(P<0.05),以缺血后72 h差异最为明显(P<0.01)。结论不完全性脑缺血后大鼠认知功能明显下降,可能与海马神经元凋亡有关。Objective To investigate the changes of cognitive function and the apoptosis of hippocampus neuron in CA1 of rats after incomplete cerebral ischemia. Methods Thirty healthy and clean male SD rats were randomly assigned into 2 groups： sham operation group（SO group,n=15） and incomplete cerebral ischemia model group（IS group,n=15）.The model of incomplete cerebral ischemia was built by bilateral carotid artery occlusion technique.The rats in SO group were only dissected to expose the blood vessels but not clip.Morris water maze equipment was used to inspect the cognitive function after ischemia for 2,3,4,5 d and 7 d respectively.Seventy-two healthy and clean male SD rats were randomly assigned into 2 groups： sham operation group（SO group,n=36） and and incomplete cerebral ischemia model group（IS group,n=36）.According to the different cerebral ischemia time,all rats were assigned into 6 groups（6 in each group）.The rats were executed,the hippocampus was extracted after ischemia for 24 h,48 h,72 h,4 d,5 d and 7 d to analyze the apoptosis rate by flow cytometry. Results Compared with SO group,the latency in IS group was prolonged at 1 d and 4 d after training,but there was no significant difference（P0.05）.At 2 d and 3 d after training,the latencies in IS group were prolonged significantly compared with SO group（P0.05）.Compared with SO group,the frequency of passing the virtual platform and the time of staying in virtual platform in IS group were less than in SO group（P 0.05）.The flow cytometry showed that the hippocampal neuron apoptosis rate showed no significant change in SO group at different time points,while the apoptosis rate in IS group began to increase at 24 h,and peaked at 72 h,then decreased gradually.The hippocampal neuron apoptosis rates were significantly higher in IS group than in SO group at different time points（P0.05）,especially at 3 d（P0.01）. Conclusion After incomplete cerebral ischemia,the decrease of cognitive function may be related with the increase of hi

关 键 词：不全脑缺血 术后认知功能障碍 学习与记忆 海马 凋亡 

分 类 号：R363[医药卫生—病理学]