Analysis of Protein Content in Panicum miliaceum L. Using Near Infrared Transmittance Spectroscopy  

近红外透射光谱技术测定黍稷蛋白含量的研究(英文)

作　　者：牛宇[1] 蒙秋霞[2] 张丽珍[3] 赵婷婷[4] 乔治军[5] 牛伟[6] 刘根科[7] 冯耐红[8] 

机构地区：[1]山西省农业科学院农业资源与经济研究所,山西太原030006 [2]山西省农业科学院农业环境与资源研究所,山西太原030006 [3]山西大学生命科学学院,山西太原030006 [4]山西大学生物工程学院,山西太原030006 [5]山西省农业科学院农作物品种资源研究所,山西太原030031 [6]山西省农业科学院,山西太原030006 [7]山西省农业科学院右玉试验站,山西右玉037200 [8]山西省农业科学院经济作物研究所,山西汾阳032200

出　　处：《Agricultural Science & Technology》2012年第5期965-968,共4页农业科学与技术（英文版）

基　　金：Supported by the National Key Technology R&D Program of China (2006BAD02B07);the National Mordern Agricultural Industry System of China(CARS-07-12.5-A12)~~

摘　　要：[Objective] To explore a method for the rapid determination of protein con- tent in grains of Panicum miliaceum L. [Method] The near infrared transmittance spec- troscopy （NITS） was used to build the mathematical models for the quantitative analy- sis of protein content in the grains. Four combinations of treatment that first derivative and second derivative were respectively combined with partial least squares （PLS） and modified partial least squares （MPLS） were set to compare their effects on the original transmission spectrum. [Result] The predicting effects of the 4 combinations were similar. The optimal combination was first derivative with MPLS, in which the average determination coefficient of validation （RSQ） was 0.880 6, correlation coeffi- cient of cross validation （1-VR） was 0.857 0, standard error of calibration （SEC） was 0.342 4, standard error of cross validation （SECV） was 0.375 1, and the standard er- ror of prediction （SEP） was 0.454. [Conclusion] The constructed NITS model is a rapid way for the determination of protein content in grains of P. miliaceum.[目的]探索快速测定完整黍稷籽粒蛋白含量的方法。[方法]采用近红外光谱分析技术建立数学模型并进行预测,比较原始透射光谱经导数处理结合不同回归算法对模型的影响。[结果]分别经一阶和二阶导数处理后利用偏小二乘法和改进的偏小二乘法,4 种方法的分析效果相近,最优的是一阶导数结合改进的偏最小二乘回归法,黍稷蛋白定标模型的定标相关系数(RSQ)为0.880 6,定标标准误差(SEC)为0.342 4,交互定标标准误差(SECV)为 0.375 1,外部预测标准误差(SEP)为 0.454。[结论]以完整黍稷籽粒为样品所建立的蛋白 NITS 模型,可以用于黍稷蛋白含量的快速检测。

关 键 词：Near infrared transmittance spectroscopy Panicum miliaceum L. PROTEIN Calibration model 

分 类 号：S516[农业科学—作物学]