胰腺癌细胞中Runx3基因表达及甲基化的研究  

The research of methylation and expression of Runx3 Gene in pancreatic cancer cell lines

作　　者：邱志东[1] 范琳峰[1] 雷长江[1] 缪辉来[1] 

机构地区：[1]广东医学院附属医院肝胆外科,广东湛江524001

出　　处：《中国实验诊断学》2012年第5期775-777,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的观察胰腺癌细胞中Runx3基因表达的情况,探讨其出现表达异常的机制。方法 (1)RT-PCR检测胰腺癌Panc-1、BxPC-3、AsPC-1细胞及正常胰腺组织中Runx3mRNA表达情况(2)Western blotting检测胰腺癌Panc-1、BxPC-3、AsPC-1细胞中Runx3蛋白表达情况;(3)甲基化特异性PCR(MSP)检测胰腺癌Panc-1、BxPC-3、As-PC-1细胞及正常胰腺组织中Runx3基因启动子区CpG岛的甲基化水平。结果 (1)Panc-1、BxPC-3、AsPC-1细胞中未检测到Runx3基因mRNA表达,而正常胰腺组织可检测到Runx3基因mRNA表达;(2)Panc-1、BxPC-3、AsPC-1细胞中未检测到Runx3蛋白表达;(3)Panc-1、BxPC-3、AsPC-1细胞中Runx3基因启动子区CpG岛均发生甲基化,而正常胰腺组织中Runx3基因启动子区CpG岛未检测到甲基化。结论胰腺癌细胞中存在Runx3基因失表达,其机制可能与Runx3基因启动子区CpG岛发生甲基化有关。Objective to observate the expression of Runx3 gene in pancreatic cancer cells,explore the mechanism about the abnormal expression of Runx3 gene.Methods（1） Runx3 mRNA expression in Panc-1,BxPC-3,AsPC-1 and normal pancreatic tissue were detected by reverse transcriptase PCR（RT-PCR）;（2） Runx3 protein expression in Panc-1,BxPC-3 and AsPC-1 was detected by Western blotting;（3） Methylation status in the CpG island of Runx3 promoter region of Panc-1,BxPC-3,AxPC-1 and normal pancreatic tissue was detected by MSP.Results（1）Runx3 mRNA expression was not detected in Panc-1,BxPC-3 and AsPC-1,but in normal pancreatic cells relative quantity of Runx3 mRNA expression was detected at high levels;（2） Runx3 protein expression were not detected in Panc-1,BxPC-3and AsPC-1;（3） CpG island of Runx3 promoter region has occurred methylation in Panc-1,BxPC-3 and AsPC-1 but has not in normal pancreatic tissue.Conclusion Runx3 gene silencing may be related to CpG island methylation status of Runx3 promoter region in pancreatic cancer cells.

关 键 词：胰腺癌 RUNX3基因 甲基化 

分 类 号：R735.9[医药卫生—肿瘤]