二株产壳聚糖酶海洋微生物的筛选和鉴定  

Screening and identification of chitosan-hydrolytic microorganisms from marine environment

作　　者：戴秋萍[1,2] 方和娣[3] 陶诗[3] 王健鑫[3] 叶兴乾[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,杭州310029 [2]浙江海氏实业集团有限公司,舟山316004 [3]浙江海洋学院海洋科学学院,舟山316004

出　　处：《海洋环境科学》2012年第3期385-390,共6页Marine Environmental Science

基　　金：浙江省水产加工创新团队项目(2009R50031-22);浙江省科技厅公益性技术应用研究项目(2011C31017);舟山市科技局海洋类项目(10248)

摘　　要：采用不同培养基对海水养殖场环境样品进行壳聚糖酶产生菌的分离,结果表明海水培养基对于菌株筛选有显著的促进作用,同时甲壳类外壳是壳聚糖酶产生菌的良好来源。KJT-A和KJT-D是从海水养殖场蟹壳表面分离到的两株壳聚糖酶产生菌株,结合形态学观察、生理生化反应和分子生物学鉴定,初步确定菌株KJT-A和KJT-D分别为假单胞菌属(Pseudo-monas)和青霉属(Penicillium)。同时分析了不同温度、盐度对两株菌株生长的影响。In this paper the chitosanase-producing microbes from the marine environment were studied. The result showed that marine medium can significantly adapt to strain isolation, and the surface of shell was best source of chitonsanase-producing bacterial strain. K.IT-A and KIT-D, which were isolated from the crab shell surface of xuwang marine culture zone in zhoushan island, were classified into Pseudomonas and Penicillium based on morphology, analysis of physiology and biochemistry and molecular biology methods. The growth of two strains in different temperature and salinity also discussed.

关 键 词：壳聚糖酶 鉴定 16S RDNA 假单胞菌 青霉 

分 类 号：Q331[生物学—遗传学]