鞘氨醇激酶通过调控血管发生促进肝癌转移  

Sphingosine kinase promote HCC metastasis via regulation of angiogenesis

作　　者：赛岩[1,2] 代学强[2] 白纪民[2] 刘勇[2] 常菁[2] 范礼斌[1] 崔春萍[2] 

机构地区：[1]安徽医科大学生命科学院生物学教研室,合肥230032 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中国医药生物技术》2012年第3期171-177,共7页Chinese Medicinal Biotechnology

基　　金：国家自然科学基金(30930041;30800558;81070330);"重大新药创制"科技重大专项(2012ZX09102301-012)

摘　　要：目的确定鞘氨醇激酶(SPK)在肝癌转移中的作用。方法用Ad-SPK1腺病毒感染肝癌细胞LM-3使其SPK过表达,Western blot检测SPK1表达及激活情况;Transwell、划痕实验检测肝癌细胞迁移情况。体外管状结构形成实验检测SPK对脐静脉内皮细胞(HUVEC)管状化形成的影响。建立SPK过表达裸鼠模型,体内检测肝癌迁移情况。结果在体外SPK对肝癌细胞的迁移无显著影响;Ad-SPK能够促进HUVEC管状结构形成;裸鼠皮下移植瘤结果显示Ad-SPK促进肿瘤生长和转移。结论鞘氨醇激酶通过调控血管发生促进肝癌转移。Objective To determine the role of sphingosine kinase （SPK） in hepatocyte carcinoma （HCC） metastasis. Methods The expression and activation of SPK in Ad-SPK infected LM-3 HCC cell line was detected by Western blot; and HCC ceils migration was evaluated by Transwell and scar-healing experiments. The impact of SPK on HUVEC tube morphologenesis was examined by in vitro tube formation assay. The SPK-overexpressing nude mice model was established to access HCC metastasis in vivo.Results SPK did not influence significantly HCC cells migration in vitro; HUVEC tube formation was stimulate by Ad-SPK. Ad-SPK accelerated the tumor growth and metastasis in vivo. Conclusion Sphingosine kinase promotes the HCC metastasis via regulating angiogenesis.

关 键 词：疾病模型 动物 肝肿瘤 肿瘤移植 移植 异种 鞘氨醇激酶 

分 类 号：R735.7[医药卫生—肿瘤]