Determination of Postvaccinal Antibodies Against OspA B. afzelii, B. garinfi and B. burgdorferi sensu stricto Using Elisa Method  

作　　者：Jiri Nepereny Vladimir Vrzal Josef Chumela 

机构地区：[1]Department of Bacterial Vaccine Research, Bioveta, a. s., Ivanovice na Hane, Komenskeho 212, 683 23, Czech Republic

出　　处：《Journal of Agricultural Science and Technology(A)》2012年第4期465-472,共8页农业科学与技术（A）

摘　　要：The specific recombinant proteins rOspA B. afzelii, rOspA B. garinii a rOspA B. burgdorferi sensu stricto were used as antigens for construction of ELISA sets. ELISA examination enables determination of specific post-vaccination antibodies against OspA B. afzelii, B. garinii a B. burgdorferi sensu stricto.Using recombinant DNA technology, genes from B. afzelii, B. garinii and B. burgdorferi sensu stricto were inserted into E. coli-expression vectors and the rOspA proteins were produced. These proteins were used for the construction of ELISA kits for the determination of post-vaccination antibodies against individual Borrelia serovars contained in the vaccine. The antibody response of dogs vaccinated with whole-cell vaccine BORRELYM 3 and non-vaccinated dogs was monitored and compared. The ELISA method proved as highly sensitive for the determination of post-vaccination antibodies against individual Borrelia serovars in vaccinated animals. Detection of these antibodies and their quantification may be used for evaluation of efficiency of vaccines against Lyme borreliosis in dogs caused by Borrelia burgdorferi sensu lato. Prospectively, it will be necessary to establish a correlation between post-vaccination antibody levels and protective immunity of vaccinated dogs.

关 键 词：BORRELIA Osp protein ELISA vaccine ANTIGEN serum 

分 类 号：S859.84[农业科学—临床兽医学] S858.3[农业科学—兽医学]