帕金森炎症细胞模型的建立与炎症机制的研究  

Study on the inflammatory mechanisms in ivtro model of Parkinson's disease

作　　者：王进华[1] 赵辉[2] 崔桂云[1] 王娇[1] 赵秋宸[3] 昝坤[1] 沈霞[1] 

机构地区：[1]徐州医学院神经病学教研室(徐州医学院附属医院神经科),徐州221000 [2]江苏徐州市中心医院神经科,徐州221000 [3]徐州医学院临床医学系,徐州221000

出　　处：《中国实用神经疾病杂志》2012年第8期16-19,共4页Chinese Journal of Practical Nervous Diseases

基　　金：江苏省卫生厅资助项目(H200926)

摘　　要：目的观察脂多糖(LPS)对小鼠中脑原代多巴胺能神经元的损伤作用。方法取孕13d的C57/BL小鼠胚胎中脑原代培养,以LPS为工具药激活小胶质细胞,通过酪氨酸羟化酶(TH)免疫细胞荧光染色,计数TH阳性细胞数目并观察形态的变化,ELASA法观察炎症因子的变化。结果 不同浓度LPS(0.1、1.0和10.0mg/L)处理72h与相同浓度LPS(1mg/L)不同时间点(8h、24h、72h)分别处理原代混合细胞多巴胺神经元,TH阳性细胞减少,大部分细胞表现为突起数目的减少,突起变短并有串珠样改变。分泌细胞因子TNF-α含量与给药组相比显著增多(P<0.001)。结论 LPS通过激活小胶质细胞释放TNF-α发挥对多巴胺能神经元的损伤作用。Objective To investigate the injury effects on dopaminergic neurons induced by LPS in mouse mesencephalic neuralgia cultures.Methods Mouse mesencephalic neuralgia cells were obtained from timed pregnant C57/BL-mouse on embryonic 13 days.Microglia were activated with LPS and then immunofluorescence.Dopaminergic neurons were identified and counted by using tyrosine hydroxylase（TH） immunochemistry.ELISA was applied to the observation of change of inflammatory factor.Results Different centrations of LPS（0.1,1.0,and 10.0 mg/L） wwere incubated for 72 hours treatment dopaminergic neurons,1.0 mg/L LPS was incubated for different times（8 h,24 h,72 h）.The number of TH positive cells reduced significantly with shorter cell processes showing bead-like.Cytokines such as TNF-α released from microglia increased greatly（P0.001）.Conclusion TNF-α may play an important role in injury effects of dopaminergic neurons,released by microglias which activated by LPS.

关 键 词：帕金森病 多巴胺神经元 脂多糖 炎症 小胶质细胞 

分 类 号：R742.5[医药卫生—神经病学与精神病学]