新生小鼠缺血缺氧性脑损伤中TLR4-TRIF信号通路的表达  

Effect of TLR-TRIF signaling during hypoxic-ischemic brain injury in neonatal mice

作　　者：黄晓雷[1,2] 崔桂云[1,2] 

机构地区：[1]徐州医学院院附属医院 神经内科 [2]徐州医学院神经病学教研室,徐州221002

出　　处：《中国实用神经疾病杂志》2012年第9期16-18,共3页Chinese Journal of Practical Nervous Diseases

摘　　要：目的建立新生小鼠缺血缺氧模型,探讨TLR4-TRIF信号通路中TLR4、IRF3的表达。方法将60只7日龄C57BL/6小鼠分为假手术组、缺血缺氧1d、2d、3d、4d、7d、建立新生小鼠缺血缺氧性脑病模型,比较鼠脑左右半球的质量,确定模型是否建立成功。以逆转录-聚合酶链反应(RT-PCR)检测大脑皮质和海马中TLR4mRNA、IRF3mRNA的表达。结果缺血缺氧1d组右侧的脑质量明显重于左侧,说明缺氧缺血1d后,右脑水肿明显。缺氧缺血组的TLR4mRNA、IRF3mRNA的表达水平均明显高于假手术组。结论脑缺氧缺血损伤可激活TLR4,引起IRF3表达量增加。Objective To observe the expressions of Toll-like receptor 4（TLR4）,IRF3 in hippocampus after hypoxic-ischemic brain damage（HIBD）in neonatal mice and analyze the correlation between them,in order to explore the role of TLR4 in the HIBD.Methods The mice were randomly divided into sham-operated group and HI group（n=10）.The models of HIBD mice were subjected to right carotidartery ligation and 10% O2 for 60 min on postnatal 7 days.The brain tissue was collected at 1st day,2nd day,3rd day,4th day,7th day.Brain was weighed in paper by electronic balance,and left and right brain weight was compared.The mRNA expressions of TLR4,IFR3 in the hippocampus after HI were detected by RT-PCR at each time point.Results Right brain of 1st day of HI weight was significantly heavier than the left brain.Compared with the sham-operated group,the mRNA expression levels of both TLR4 reached a peak at 1st day and then decreased gradually.IRF3 began to increase at 1st day and reached a peak at 2nd day and then gradually declined,but had no statistical difference at 7th day（P0.05）.Conclusion The expressions of TLR4 and IRF3 in cerebral cortex and hippocampus after hypoxic-ischemic brain damage in neonatal mice are elevated,which may play a critical role in the mechanism of hypoxic-ischemic brain injury in neonatal mice.

关 键 词：缺氧缺血性损伤 TOLL样受体4 海马 小鼠 

分 类 号：R-332[医药卫生]