树突状细胞和白细胞介素23与溃疡性结肠炎的关系  

Relationship between Dendritic cell, Interleukin-23 and Ulcerative Colitis

作　　者：江勇[1] 吕宗舜[2] 

机构地区：[1]天津医科大学第二医院消化内科,300211 [2]天津医科大学总医院消化内科

出　　处：《中国综合临床》2012年第6期630-633,共4页Clinical Medicine of China

摘　　要：目的探讨溃疡性结肠炎（UC）与肠易激综合征（IBS）患者肠黏膜组织中$100阳性表达的树突状细胞（S100^＋DC）、CD83阳性表达的树突状细胞（CD83^＋DC）与白细胞介素（1L）23P19的表达差异。方法以60例UC、60例IBS患者的结肠镜活检组织蜡块标本为研究对象，应用免疫组化SP法检测两种病变肠黏膜组织中S100^＋DC、CD83^＋DC及IL-23P19的表达情况。结果两种病变组织中S100^＋DC、CD83^＋DC阳性表达率差异无统计学意义（P〉0．05）；IL-23P19阳性表达率差异有统计学意义（85．O％与70．O％，）（2=3．87，P〈0．05）；轻型UC组S100^＋DC、CD83^＋DC及IL-23P19三个指标阳性细胞浸润密度分别为（24．43±8．26）、（5．23±1．66）、（95．03±18．39）个／mm^2，重型UC组分别为（36．13±11．36）、（10．13±3．29）、（133．38±25．32）个／mm^2，两组对应的指标比较差异均有统计学意义（P均〈0．05）；全结肠型与局部结肠型UC的三个指标阳性细胞浸润密度比较，差异均无统计学意义（P均〉0．05）。结论UC组织中DC的浸润密度增高且分化能力增强，IL一23P19分泌增多；UC组织中，随着病情的加重S100^＋DC、CD83^＋DC、IL-23P19阳性表达明显增多。Objective To compare the levels of S100^＋ DC, CD83^＋ DC and IL-23P19 between the patients with mucosa of ulcerative colitis （ UC ） and those with irritable bowel syndrome （ IBS ）. Methods Paraffin-embedded colon tissue from colonoscopie biopsy was detected in sixty UC and sixty IBS cases for the positivity of S100^＋ DC, CD83^＋ DC and IL-23P19 using immunohistochemical SP method. Results There was no significant difference in the positive rates of S100^＋ DC and CD83^＋ DC between the.two groups （ P 〉 0. 05 ）, while significant difference in IL-23P19 level was found between the two groups （ 85.0% vs. 70. 0% ; X^2 = 3.87, P 〈 0. 05 ）. The infiltration densities of S100^＋ DC, CD83^＋ DC and expression of IL-23P19 in the mild and severe UC patients were 24.43 ± 8. 26/mm2,5.23 ± 1.66/mm^2, 95.03 ± 18. 39/mm^2, and 36. 13 ± 11.36/mm^2, 10. 13 ± 3.29/mm^2, 133.38 ± 25. 32/mm^2, respectively. The difference between the mild and severe UC patients was significant （ P 〈 0. 05 ） . No significant difference was found regarding the above three parameters between severity-stratified subgroups of the UC patients （ P 〉 0. 05 ）. Condusion In UC colon tissues, the infiltration density and activity of DC were increased. IL-23P19 level was elevated. The levels of S100^＋ DC, CD83^＋ DC and IL-23P19 were increased with the advancement of UC disease.

关 键 词：溃疡性结肠炎 肠易激综合征 树突状细胞 白细胞介素23 免疫组化 

分 类 号：R574.62[医药卫生—消化系统]