内异症不孕患者种植窗期子宫内膜组织中差异蛋白的表达及其与子宫内膜容受性的关系  

Study on the relationship between altered expression of annexin A4 and endometrial receptivity during the implantation window in infertile patients with endometriosis

作　　者：蒋亚玲[1] 李冰[1] 邢福祺[2] 王芳[1] 冯健怀[1] 

机构地区：[1]广州医学院第三附属医院妇产科,510150 [2]南方医科大学南方医院生殖医学中心

出　　处：《中华妇产科杂志》2012年第5期324-327,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：2011年广州医学院第三附属医院青年科研支持计划项目（2011Y04）

摘　　要：目的探讨内异症不孕患者种植窗期子宫内膜组织中差异蛋白的表达及其与子宫内膜容受性的关系。方法采用荧光差异凝胶电泳（2D—DIGE）和基质辅助激光解吸离子化飞行时间质谱（MALDI-TOF—MS）技术，对因输卯管因素不孕接受腹腔镜治疗的非内异症患者（10例，对照组）和内异症（术后病理检查证实）不孕患者（10例，内异症组）种植窗期（排卵日后第6天）在位子宫内膜的差异表达蛋白进行筛选并鉴定；应用蛋白印迹法检测两组患者子宫内膜组织中表达差异最明显的膜联蛋白A4蛋白的表达变化，探讨膜联蛋白A4与子宫内膜容受性的关系。结果两组患者种植窗期子宫内膜有意义（上调或下调150％）的差异表达蛋白共7个，其中等电点为5．84，相对分子质量为36100的蛋白在内异症组患者种植窗期的表达量较对照组减少348％，为差异表达最明显的蛋白；经鉴定，此蛋白斑点肽指纹图谱为膜联蛋白A4，其蛋白相对表达量内异症组为7．2±0．9，对照组为17．8±2．6，两组比较，差异有统计学意义（t=7．654，P=0．002）。结论内异症不孕患者种植窗期子宫内膜存在多种差异表达蛋白，其中膜联蛋白A4蛋白表达量下调最显著，可能与子宫内膜对胚胎的容受性下降有关。Objective To identify the differential expressed proteins, and to investigate the relationship between altered expression of annexin A4 during window of implantation [ WOI （ at day-6 after ovulatory day ）] in infertile patients with endometriosis and endometrial receptivity. Methods Two- dimensional fluorescence differential in-gel electrophoresis （2D-DIGE） and matrix-assist laser desorption ionization-time of flight-mass spectrometry （MALDI-TOF-MS） were used to detect protein expression in endometrial WOI in 10 infertile cases with endometriosis as endometriosis group and 10 infertile cases with tuba] factors as control group. The semi-quantitative validation of annexin A4 in the eutopie endometrial tissue during WOI was analyzed by western blot. Results By comparing protein profiles, there were 7 meaningful differential proteins during WOI in infertile patients with endometriosis. One protein with an isoelectric point of 5.84 and relative molecular weight of 36 100 were down regulated 348% in samples of endometriosis group. It was identified as annexin A4 by mass spectrometry. By western blot, relative intensity of annexin A4 in endometriosis group was 7.2 ± 0. 9, which was lower than 17.8 ± 2. 6 in control group significantly （t = 7. 654, P = 0. 002 ）. Conclusion Lower expression of annexin A4 during WOI in infertile patients with endometriosis might be associated with the decrease of endometrial receptivity.

关 键 词：子宫内膜异位症 不育 女(雌)性 膜联蛋白A4 胚胎植入 子宫内膜 蛋白质组学 

分 类 号：R711.6[医药卫生—妇产科学]