基于MR血管成像比较两种兔蛛网膜下腔出血后脑血管痉挛模型  

Comparison on two different hemorrhage rabbit models of cerebral vasospasm after subarachnoid hemorrhage using MR angiography

作　　者：朱友志[1] 张禹[1] 骆祥伟[1] 李大圣[1] 莫子[1] 张子轩[2] 邓雪飞[2] 韩卉[2] 

机构地区：[1]中国人民解放军105医院影像科,安徽合肥230031 [2]安徽医科大学人体解剖学教研室,安徽合肥230032

出　　处：《中国介入影像与治疗学》2012年第5期380-384,共5页Chinese Journal of Interventional Imaging and Therapy

基　　金：国家自然科学基金面上项目(30771137)

摘　　要：目的应用MRA对比观察一次注血法和二次注血法制造兔蛛网膜下腔出血后脑血管痉挛模型的差异。方法新西兰兔40只,随机分为对照组(5只)、一次注血组(17只)和二次注血组(18只),分别以生理盐水(对照组)及自体动脉血(注血组)注入枕大池。于造模前和造模后1、3、5、7、9、11天行神经功能评分,采用MRA技术观测基底动脉管径的变化情况。结果 3组动物死亡率差异无统计学意义(均P>0.05)。对照组无明显神经功能损伤,基底动脉管径造模前后差异无统计学意义(均P>0.05)。一次注血组造模后3天神经功能损伤最明显,基底动脉于造模后1天发生收缩痉挛,于造模后3天痉挛达到高峰,管径为造模前的68.18%,后逐渐恢复正常。二次注血组造模后5天神经功能损伤症状最明显,基底动脉于造模后1天发生收缩痉挛,于造模后5天痉挛达到高峰,管径为造模前的56.72%,后逐渐恢复正常。结论兔枕大池二次注血法是制造蛛网膜下腔出血后脑血管痉挛模型的良好方法;采用MRA技术可活体观测脑血管痉挛。Objective To compare the cerebral vasospasm after subarachnoid hemorrhage （SAH） between one- and twohemorrhage rabbit models using MR angiography （MRA）. Methods A total of 40 rabbits were randomly divided into control group （n~ 5）, one （n= 17）- and two （n= 18） hemorrhage model groups. The hemorrhage models were made by infusing autologous arterial blood into the cisterna magna, while rabbits in control group were injected with normal saline. The diameter changes of basilar artery （BA） were observed using MRA before and after modeling （1, 3, 5, 7, 9, 11 days）. Nerves-functional evaluation was performed before MRA examination in all rabbits. Results There was no statistical difference in mortality among three groups （all P〉0.05）. The neurologic score and the mean diameter of BA were not statistically different （all P〉0.05） 0--11 days in control group. In the one-hemorrhage group, the neurological damage appeared mostly after 3 days. The diameter of BA became narrow 1 day after modeling, reaching the most narrowing 3 days later, with the diameter decreased to 68.18%, and then gradually recovered. In the two-hemorrhage group, the neurological damage appeared mostly in 5 days. The diameter of BA became narrow 1 day after modeling, reaching the most narrowing 5 days later, with the diameter decreased to 56.72%, and then gradually recovered. Conclusion The two-hemorrhage with autologous arterial blood into the cisterna magna is a reliable method for the research on cerebral vasospasm in rabbits. MRA can be used for in vivo imaging investigation for cerebral vasospasm.

关 键 词：蛛网膜下腔出血 血管痉挛 颅内 动物模型 磁共振血管成像 

分 类 号：R743.35[医药卫生—神经病学与精神病学]