膀胱癌组织中MICA、NF—κB和p53的表达和意义  

Expression and significance of MICA, NF-κB and P53 in carcinoma of the urinary bladder

作　　者：邓汪东[1] 卢善明[3] 杨峰涛[2] 

机构地区：[1]中山大学附属梅州医院泌尿外四科,广东省梅州514031 [2]汕头大学医学院第一附属医院泌尿外科 [3]中山大学附属梅州医院病理科

出　　处：《中国医师杂志》2012年第4期445-448,共4页Journal of Chinese Physician

基　　金：基金项目：广东省科技计划项目资助（20108031600279）;广东省医学科研基金资助项目（A2010398）

摘　　要：目的探讨膀胱尿路上皮癌中MHCI类链相关蛋白A（MHC class I chain．relatedA，MICA）的表达，以及与核因子-KB（ntlclear factor-κB，NF—κB）和p53的相互关系，为研究膀胱癌组织中MICA蛋白的表达机制提供组织学依据。方法用免疫组化方法检测75例膀胱尿路上皮癌及15例正常膀胱黏膜中MICA、NF-κB和p53蛋白表达，对MICA、NF-κB和p53在正常膀胱黏膜、浸润和非浸润膀胱癌中的表达进行统计学分析。结果（1）MICA、NF—κB和p53蛋白在膀胱癌的表达率分别为48．0％、85．3％和49．3％，均显著高于正常膀胱黏膜（P〈0．05）。MICA蛋白在浸润性膀胱癌的表达低于非浸润性膀胱癌（P〈0．05）。（2）膀胱癌组织中MICA与NF-κB蛋白表达呈正相关（r=0．256，P=0．027），而MICA和p53蛋白的表达呈负相关（r=-0．23，P=0．047）。结论膀胱癌中MICA蛋白常表达上调，可作为膀胱癌的肿瘤相关抗原；NF-κB通路可能参与MICA的表达调控；p53通路可能不参与膀胱尿路上皮恶性转化过程中MICA蛋白的表达。Objective To investigate the mechanism of the expression of MHC class I chain-related A （MICA） in carcinoma of the urinary bladder, the relationship between MICA, nuclear factor-κB （NF- κB） and p53 expression in bladder cancer was studied. Methods The expression of MICA, NF-κB and p53 in a total of 75 cases of urothelial carcinoma tissues and 15 normal mucous membrane tissues of the bladder was evaluated by immunohistochemistry. Results The expression rates of MICA, NF-κB and p53 protein in urothelial carcinoma were 4.08%, 85.3% and 49. 3 %, respectively. Up-regulation of their expression was found in urothelial carcinoma compared with normal mucous membrane tissues （ P 〈 0. 05 ）. MICA expression was positively correlated with NF-κB expression（ r =0. 256, P =0. 027）, but negatively correlated with p53 expression（ r = - 0. 23, P = 0. 047）. Conclusions Up-regulation of MICA expres- sion was detected in bladder cancer. MICA protein may be a new tumor-associated antigen of bladder cancer. MICA expression may be regulated by NF-κB pathway, while p53 pathway may not play a part in MICA up-expression during the urothelial malignant transformation.

关 键 词：膀胱肿瘤/病理学/代谢 组织相容性抗原I类/代谢 NF-κB/代谢 肿瘤抑制蛋白质p53/代谢 

分 类 号：R737.14[医药卫生—肿瘤]