细胞转录因子E2F-1及其转录靶向分子双特异性磷酸酶PACl诱导人骨肉瘤细胞凋亡的作用  

Roles and mechanisms of E2F-1 and PAC1 in signaling oxidative stress-induced apoptosis of Saos-2cells

作　　者：吴晶晶[1] 樊慧杰[1] 樊青霞[1] 张明智[1] 

机构地区：[1]郑州大学第一附属医院肿瘤科,450052

出　　处：《中华医学杂志》2012年第17期1219-1221,共3页National Medical Journal of China

摘　　要：目的研究细胞转录因子E2F-1及其转录靶向分子双特异性磷酸酶（PAC-1）在高氧刺激下诱导人骨肉瘤细胞Saos-2凋亡的作用及机制。方法利用小干扰RNA技术构建siE2F-1和siPACl的细胞克隆，锥虫蓝染色法观察不同E2F-1或PACl表达水平的Saos-2细胞在高氧刺激后细胞存活率的变化，并通过Western印迹法检测其下游相关凋亡靶分子的表达水平。结果细胞Saos．2／siE2F-1和Saos-2／siPACl经H2O2刺激后相对于对照组存活率均明显提高，其内p-ERKl／2的表达相对于对照组保持在一个较高的水平，其余被检测的靶分子没有看到明显被诱导表达的现象。结论E2F-1／PAcl／MAPKase途径是一条特异性的传递凋亡信号的通路。Objective To explore the roles and mechanisms of E2F-1 and PAC1 in signaling apoptosis of Saos-2 ceils under oxidative stress. Methods siRNAs were used to construct the cell clones of expressing siE2F-1 and siPAC1. E2F-1/PACl-mediated induction of apoptotic cell death in response to H202 were examined by trypan blue exclusion and the expression levels of related target factors detected by Western blot. Results The cell viabilities of Saos-2/siE2F-1 and Saos-2/siPAC1 increased markedly comparing with the control cells after the treatment of H202. And the expression level of p-ERK1/2 was higher than that of the control cells. Conclusions The pathway of E2F-1/PAC1/MAPKase is a specific cascade for apoptotic signaling.

关 键 词：骨肉瘤 高压氧 细胞凋亡 

分 类 号：R738[医药卫生—肿瘤]