原花青素对树脂-牙本质粘接界面耐冷热循环老化作用的影响  

The potential effect of proanthocyanidins on the stability of resin-dentin bonds against thermal cycling

作　　者：刘瑞瑞[1] 方明[1] 赵三军[1] 李芳[1] 沈丽娟[1] 陈吉华[1] 

机构地区：[1]第四军医大学口腔医学院修复科,西安710032

出　　处：《中华口腔医学杂志》2012年第5期268-272,共5页Chinese Journal of Stomatology

基　　金：基金项目：国家自然科学基金（81000458、81130078）

摘　　要：目的探讨天然交联剂原花青素预处理脱矿牙本质是否对冷热循环下树脂．牙本质粘接界面有保护作用。方法制备10％和15％原花青素交联预处理剂、5％戊二醛用于脱矿牙本质粘接前的短暂预处理（60和120s），无预处理组为阴性对照。根据冷热循环与否分亚组，每亚组4颗牙，制备树脂一牙本质粘接试件，即刻或于冷热循环（5000次）后测试微拉伸粘接强度，观察粘接断裂模式、界面微观形貌并测量胶原降解量。结果冷热循环后15％原花青素预处理60和120s组粘接强度[分别为（21．88±3．49）和（23．09±3．19）MPa]显著高于阴性对照组[（15．47±3．78）MPa]（P〈0．05）。各组粘接断裂模式均以混合破坏和界面破坏为主。各预处理组粘接界面混合层均一致密，而阴性对照组混合层中可见狭窄的空隙或裂缝。所有预处理组冷热循环后胶原降解量均显著低于阴性对照组（P〈0．05），其中15％原花青素预处理120S组胶原降解量最低[（0．316±0．019）mg／g]。结论应用原花青素预处理脱矿牙本质可减缓因冷热循环引起的树脂．牙本质粘接界面退变，有助于提高粘接修复体的耐久性。Objective To investigate the potential effect of proanthocyanidins （ PA ）, a natural cross-linker, on the stability of resin-dentin bonds against thermal cycling. Methods Ten percent, 15% PA-based preconditioners, and 5% glntaraldehyde were prepared for the transient pretreatment of demineralized dentin before bonding. Specimens without pretreatment were used as negative controls （n = 4 teeth for each group）. Microtensile bond strength, failure mode, micromorphologies of resin-dentin interface and the collagen degradation of bonded specimens after thermal cycling were evaluated. Results After thermal cycling, the microtensile bond strength values of resin-dentin bond in groups pretreated with 15% PA for 120 s and 60 s [ （23.09 ± 3.19） and （21.88 ± 3.49） MPa] were significantly higher than that in control group [ （ 15.47 ± 3.78） MPa] （P 〈0. 05）. Mixed fractures were the most prevalent failure mode. Specimens with pretreatment presented compact hybrid layer, while some narrow gaps were found in hybrid layer of non-treated specimens. Collagen biodegradation rates in groups with pretreatment were significantly lower than that in control group （P 〈 0. 05 ）. Among them, specimens pretreated by 15% PA preconditioner for 120 s exhibited the lowest biodegradation rates [ （0. 316 ± 0. 019） mg/g]. Conclusions The application of natural cross-linker PA on demineralized dentin reduced the bond degradation against aging by thermal cycling, and can be helpful to create more durable bonds to dentin.

关 键 词：原花青素类 牙本质粘结剂 胶原 拉伸强度 

分 类 号：R783.1[医药卫生—口腔医学]