慢性间歇低氧大鼠模型在体舌下神经电信号记录实验平台的建立  

Construction of An Experimental Hypoglossal Nerve Signals Record Platform in Rat Model of Chronic Intermittent Hypoxia in Vivo

作　　者：赵景景[1] 文芳静[1] 王念[1] 范慧[1] 万志辉[1] 胡克[1] 

机构地区：[1]武汉大学人民医院呼吸内科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2012年第3期350-354,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81070065)

摘　　要：目的:建立一种以模拟阻塞性睡眠呼吸暂停低通气综合征的慢性间歇低氧大鼠模型为基础、探讨在体舌下神经电信号活动的实验平台。方法:慢性间歇低氧大鼠模型由气源(氮气及空气)、电磁阀控制系统、间歇低氧动物仓、以及实验动物(大鼠)组成,按照实验要求对大鼠交替给予氮气和空气,每天8h,连续处理7周后,分离舌下神经,应用多通道生物信号采集与处理系统对舌下神经电信号进行处理与分析。结果:预实验结果显示,慢性间歇低氧大鼠模型能够模拟人体阻塞性睡眠呼吸暂停低通气综合征的病理生理特征,所记录的电信号稳定、可靠。结论:慢性间歇低氧大鼠模型简单、易行、实用、重复性好,记录的舌下神经电信号稳定、可靠,达到实验设计要求。Objective： To establish an experimental platform of the rat model of chronic intermittent hypoxia simulating obstructive sleep apnea syndrome and to explore the activity of the hypoglossal nerve signals in vivo.Methods： Experiments were performed in the rat model of chronic intermittent hypoxia.These preparations included the gas source,the gas control delivery system,the intermittent hypoxia animal chambers,and the rats.In accordance with the requirement of experiment,the rats were given nitrogen and air alternately for 8 hours per day and the experiment lasted for 7 weeks.After the experiment,the hypoglossal nerve was isolated and the average peak amplitude and frequency of hypoglossal nerve discharges were recorded and analyzed with medical biological signal processor（MedLab U/C 501H）.Results： The pre-test results showed that chronic intermittent hypoxia rat model could simulate the pathophysiological characteristics of the obstructive sleep apnea hypopnea syndrome in human,and the recorded signals from hypoglossal nerve are stable and reliable.Conclusion： The chronic intermittent hypoxia model proved simple,easy,practical,and reproducible,and the recorded signals of hypoglossal nerve are stable and reliable.The system is ideally suited for studies.

关 键 词：慢性间歇低氧 阻塞性睡眠呼吸暂停低通气综合征 舌下神经 动物模型 

分 类 号：R363.21[医药卫生—病理学]