曲古抑素A预处理对大脑中动脉闭塞模型大鼠脑保护作用及其与IL-1β的关系  

Cerebral protection of Trichostatin A preconditioning in rats with middle cerebral artery occlusion and the relationship between Trichostatin A and IL-1β

作　　者：贺民[1] 郭曲练[1] 

机构地区：[1]中南大学湘雅医院麻醉科,长沙410078

出　　处：《中南大学学报（医学版）》2012年第4期379-383,共5页Journal of Central South University ：Medical Science

摘　　要：目的：探讨曲古抑素A（TSA）预处理对大脑中动脉闭塞（MCAO）模型大鼠的脑保护作用及其与IL-1β之间的关系,并评价年龄对TSA预处理所产生大鼠脑保护作用的影响。方法：采用梭性头端线栓法制备局灶性脑缺血再灌注模型,将96只SD青年雄性大鼠（3-4月龄）随机分为缺血再灌注对照组、二甲基亚砜（DMSO）预处理组、小剂量TSA预处理组（0.03 mg/kg）和大剂量TSA预处理组（0.1 mg/kg）,每组包括缺血再灌注6 h组、12 h组、24 h组、48 h组,每组6只大鼠。另将18只SD雄性大鼠分为老年组（22月龄）、青年组（3~4月龄）、幼年组（1月龄）,均采用大剂量TSA预处理。采用方差分析比较不同处理组脑梗死体积比、脑脊液和血液IL-1β含量的差异,Spearman秩相关分析脑梗死体积比与脑脊液、血液IL-1β含量的线性相关。结果：大剂量TSA组大鼠脑梗死体积比在缺血再灌注各时间点均小于其余3组。大剂量TSA组缺血再灌注各时间点脑脊液中IL-1β含量与对照组相应时间点相比明显减少。大剂量TSA组血清中IL-1β含量在缺血再灌注各时间点均低于DMSO组和对照组,小剂量TSA组与DMSO组无差别。脑梗死体积与血液IL-1β、脑脊液IL-1β含量的相关系数分别为0.841和0.618（P〈0.05）。不同年龄组大鼠的脑梗死体积比较差异无统计学意义,但老年组大鼠脑梗死体积大于其余两组。不同年龄组大鼠脑脊液中的IL-1β含量差异无统计学意义（P=0.076）。结论：大剂量TSA预处理可减小脑梗死体积比,并降低脑脊液和血液IL-1β含量;年龄对大剂量TSA预处理大鼠脑梗死体积比、血液和脑脊液IL-1β没有影响。Objective：To explore the impact on cerebral protection of Trichostatin A（TSA） preconditioning in rats with middle cerebral artery occlusion（MCAO）;the relationship between cerebral protection of TSA and interleukin-1 beta（IL-1β）;and the impact of age on the mechanism of cerebral protection of TSA.Methods：The modified suture method was used to create stable a MCAO model.A total of 96 male SD rats were assigned randomly to four groups：a control group,a dimethyl sulfoxide（DMSO） preconditioned group,a low-dose（0.03 mg/kg） TSA-preconditioned group,and a high-dose（0.1 mg/kg） TSA-preconditioned group.Each group included four sub-groups for reperfusion for 6,12,24 and 48 hours,respectively,6 rats per sub-group.An additional,eighteen healthy,male Sprague Dawley（SD） rats that received TSA preconditioning（0.1 mg/kg） were divided into three groups based on their age：young,mid-age,and old,One-way analysis of variance was used to compare the differences between groups,and the Spearman rank correlation was used to examine relationships between IL-1β concentrations in blood and cerebrospinal fluid and cerebral infarction volume.Results：The cerebral infarction volume of rats in the high-dose TSA group was less than that of the other 3 groups（P0.05）.The IL-1β in blood and the cerebrospinal fluid of rats in the high-dose TSA group was lower than that in control and DMSO groups（P0.05）;for the low-dose TSA group IL-1β levels were statistically the same as in controls.The Spearman rank coefficients were 0.841 and 0.618 for cerebral infarction volume correlate to blood IL-1β and to cerebrospinal fluid IL-1β,respectively（P0.05）.No statistical differences were found in cerebral infarction volume and IL-1β levels in blood or cerebrospinal fluid（P0.05）.Conclusion：High-dose TSA preconditioning reduces cerebral infarction volume and decreases IL-1β levels in blood and cerebrospinal fluid;age does not affect these parameters.

关 键 词：曲古抑素A 大脑中动脉闭塞 IL-1Β 年龄 

分 类 号：R743.3[医药卫生—神经病学与精神病学]