体外诱导翼状胬肉成纤维细胞向平滑肌细胞的分化  

Induced differentiation of pterygium fibroblasts into smooth muscle cells in vitro

作　　者：石颖颖[1] 李永平[1] 张文忻[1] 郑建樑[1] 林建贤[1] 

机构地区：[1]中山大学中山眼科中心国家重点实验室病理室,广东省广州市510060

出　　处：《中国组织工程研究》2012年第14期2589-2592,共4页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(30672276)~~

摘　　要：背景:作者前期研究发现翼状胬肉组织中存在平滑肌成分,但此平滑肌的来源并不清楚。目的:探讨翼状胬肉组织中平滑肌成分的起源。方法:组织块法对26例翼状胬肉进行原代细胞培养及鉴定。生长状态较好的原代细胞传代后分为诱导组和对照组,分别置于含或不含20μg/L碱性成纤维细胞生长因子的培养基中培养。倒置显微镜下对比观察两组细胞的形态学变化;共聚焦显微镜下观察免疫荧光染色波形纤维蛋白和α-平滑肌肌动蛋白在两组细胞中的表达情况。结果与结论:免疫组织化学染色显示所有细胞均表达波形纤维蛋白,鉴定为成纤维细胞。倒置显微镜下观察:对照组细胞呈长梭形,编织状排列;诱导组细胞饱满,胞体增大,呈"峰-谷样"生长。共焦显微镜观察:对照组细胞均表达波形纤维蛋白,不表达α-平滑肌肌动蛋白;诱导组细胞除表达波形纤维蛋白外,还不同程度地表达α-平滑肌肌动蛋白,胞浆内可见伸向细胞两极的肌动蛋白丝,证实为平滑肌细胞。结果表明翼状胬肉来源的成纤维细胞在体外经碱性成纤维细胞生长因子诱导可以向平滑肌细胞分化。BACKGROUND：Our previous findings have demonstrated that smooth muscle exists in pterygium,but its origin remains unclear.OBJECTIVE：To investigate the origin of smooth muscle in pterygium.METHODS：Pterygial tissue was harvested from 26 patients.Cells were primary cultured and identified.Cells in good growth state were divided into two groups after first reproducement,and then subcultured in the medium with or without 20 μg/L basic fibroblast growth factors（induction group and control group）.Morphological change of cells in two groups was observed through the use of inverted microscope.The expression of vimentin and α-smooth muscle actin in cells in two groups was detected by immunofluorescence.RESULTS AND CONCLUSION：Immunohistochemical staining showed that all cells expressed vimentin and were identified as fibroblasts.Under inverted microscope,cells from the control group exhibited shuttle-shaped appearance and weaved together,and cells from the induction group became plump with large cytones and grew in a ＂peak-valley＂ way.Confocal microscopy showed that in the control group,cells expressed vimentin,but did not express α-smooth muscle actin;in the induction group,cells expressed α-smooth muscle actin to different extents besides vimentin and exhibited myofilament in the cytoplasm,confirming smooth muscle cells.These findings indicate that fibroblasts from pterygium can differentiate into smooth muscle cells after induced by basic fibroblast growth factors in vitro.

关 键 词：翼状胬肉 成纤维细胞 碱性成纤维细胞生长因子 平滑肌 起源 

分 类 号：R394.2[医药卫生—医学遗传学]