高分化星形细胞瘤的蛋白质组学研究  

Proteomic study of well-differentiated astrocytoma

作　　者：肖惠生[1] 熊光仲[2] 路俊仙[3] 梁宋平[3] 袁峰[2] 

机构地区：[1]厦门市第三医院神经外科,厦门361100 [2]中南大学湘雅二医院神经外科,410011 [3]湖南师范大学生命科学院,410081

出　　处：《临床肿瘤学杂志》2012年第4期310-316,共7页Chinese Clinical Oncology

摘　　要：目的研究高分化星形细胞瘤差异蛋白质表达,为星形细胞瘤的治疗及预后的判断提供依据。方法取经病理证实的29例正常脑组织及36例高分化的星形细胞瘤(KernohanⅡ级),经蛋白电泳、染色,采用PDQUEST和2-DE分析系统软件进行分析。以MALDI-TFO质谱或MALDI-TOF/TOF串联质谱技术结合数据库检索对蛋白质进行鉴定。结果通过双向电泳得到正常脑组织和高分化星形细胞瘤标本的双向凝胶电泳图谱;生物质谱技术鉴定了24个差异蛋白质点,与正常脑组织相比,高分化星形细胞瘤有9个蛋白质下调,15个蛋白质上调。结论以蛋白质组学技术鉴定了正常脑组织和高分化星形细胞瘤的差异蛋白质,其中部分蛋白质有助于深入研究星形细胞瘤的发生、发展机制并对进一步发现肿瘤标记物及治疗靶点有重要的参考价值。Objective To study the differential proteins of well-differentiated astrocytoma and identify tumor-associated protein markers for diagnosis and prognosis.Methods The specimen of normal brain tissue（29 cases） and well-differentiated astrocytoma（36 cases,Kernohan II） identified by pathological method were electrophoresed and dyed,and then were analyzed by PDQUEST and two-dimentional electrophoresis（2-DE） softwares.The proteins were identified by MALDI-TOF mass spectrometry or MALDI-TOF/TOF tandem mass spectrometry.Results We obtained 2-DE gel atlas of normal brain and well-differentiated astrocytoma by 2-DE and 24 prominent different proteins spots by mass spectrometry.Compared with normal brain tissue,9 down-regulated and 15 up-regulated proteins were found in astrocytoma.Conclusion We have got different proteins from normal brain and well-differentiated astrocytoma by proteomics.Some proteins are useful for discovering the molecular mechanisms of genesis development and helpful to find the markers of tumor and treatment target.

关 键 词：蛋白质组学 双向凝胶电泳 质谱分析 MALDI-TOF/TOF串联质谱 胶质瘤 星形细胞瘤 

分 类 号：R730.264[医药卫生—肿瘤]