印度块菌、夏块菌与化香树合成菌根苗技术初探  

Techniques for the Synethesis of Mycorrizal Seeding of Platycarya strobilacea with Tuber indicum and T. aestivum

作　　者：苏开美[1] 李树红[1] 杨丽芬[1] 赵静[1] 赵永昌[1] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,昆明650221

出　　处：《中国食用菌》2012年第3期10-12,共3页Edible Fungi of China

基　　金：国家现代农业产业技术体系(CARS-24)经费资助

摘　　要：印度块菌(Tuber indicum Vittadini)、夏块菌(T.aestivum Vittadin)i是经济价值较高的菌根食用菌。本文对印度块菌、夏块菌与化香树(Platycarya strobilacea Sieb.et Zucc).合成菌根苗的技术进行了研究,结果表明用印度块菌、夏块菌孢子液接种化香树苗后,第三个月发现已形成根尖淡乳色,基部淡黄色、黄褐色的外生菌根。外生菌根为单轴状、羽状和不规则分支状,单根菌根长300μm^4 000μm,直径为200μm^500μm;平坦或从菌根延伸出外延菌丝。印度块菌与化香树形成的外延菌丝为透明、半透明、絮状。夏块菌与化香树形成的外延菌丝为黄绿色、羊毛状。菌根老化后变暗褐或萎缩;两种块菌与化香树形成的菌根在显微镜下有明显的差别。Tuber indicum and T. aestivum are mycorrizal mushroom with high economic value. In this paper, the production technique for the mycorrhizal seeding of Platycarya strobilacea with T. indicum and T. aestivum was studied. The result showed that T. indicum and T. aestivum formed bristle ectomycorrhizae on the rootlet tip with yellowish to yellowish brown on the Platycarya strobilacea seeding in three months. Ectomycorrhizae were monopodial, pinnate or anomalous, 300 μm- 500 μm in diameter and 300μm-4 000 μm in length. The fungus mantle was mooth or with semi-transparent, extrametri cal, flocculent. The extrametrical mycelia of mycorrhizae formed with Tuber indicum were transparent, semi-transparent, flocculent and those with T. aestivum were green-yellow, flocculent.The aging mycorrhizae become russety, wilted and shrunken. The obvious difference bwtween two ectomycorrhizae in microscopic structure could be observed.

关 键 词：印度块菌 夏块菌 化香树 显微结构 无菌苗 共生真菌 

分 类 号：S646.9[农业科学—蔬菜学]