固定化铜绿假单胞菌生物降解对硝基苯酚  

Biodegradation of p-Nitrophenol by Immobilized Cells of Pseudomonas aeruginosa

作　　者：黄强[1] 张明强[2] 

机构地区：[1]厦门大学嘉庚学院,福建漳州363105 [2]漳州师范学院化学与环境科学系,福建漳州363000

出　　处：《环境工程技术学报》2012年第3期247-252,共6页Journal of Environmental Engineering Technology

基　　金：国家水体污染控制与治理重大专项(2009ZX07317-003)

摘　　要：用海藻酸钠作载体,将一株能以对硝基苯酚(PNP)为唯一碳源和氮源的铜绿假单胞菌(Pseudomonas aeruginosa)进行固定化包埋,利用正交试验确定该菌株固定化细胞制备的最优条件,探讨固定化细胞技术对PNP的生物降解效果及其影响因素。结果表明,该菌株的固定化细胞对PNP的生物降解速度大于游离细胞;固定化细胞对PNP的耐受浓度比游离细胞高;固定化细胞生物降解PNP的最适pH为8.0～9.0,最适温度为30～35℃。该固定化细胞在PNP浓度为50mg/L的无机盐溶液中培养42h,可使PNP降解率接近100%,其生物降解的时间动力学曲线分为0～35h和35～42h两段,其中0～35h为生长延滞期,35～42h随着菌株生长的开始,PNP迅速被生物降解,约7h生物降解完全结束。该菌株的固定化细胞可连续使用3次,生物降解效果稳定。Pseudomonas aeruginosa which can grow with p-nitrophenol as sole carbon and nitrogen sources was immobilized in sodium alginate. Optimal preparation condition of the strain immobilization was determined by the cross-test experiments, and the effects and influencing factors of immobilized cells technique on the degradation of p-nitrophenol studied. The results show that the degradation efficiency of immobilized cells is apparently superior to that of free cells. The tolerant concentration of immobilized cells is higher than that of free cells. The optimal pH of degrading p-nitrophenol is 8.0-9.0, and the optimal temperature is 30-35 ~C. The removal rate of p-nitrophenol at the concentration of about 50 mg/L approaches 100% after 42 h. The curve of the kinetics of the degradation can be divided into two parts. The first part is lag period from zero hour to 35 hours and the second part is from 35 hours to 42 hours, p-nitrophenol can be degraded rapidly within 7 hours with the growth of the strain. Immobilized cells can be used to degrade p-nitrophenol repeatedly and continuously for three times and can keep steady degradation ratio.

关 键 词：对硝基苯酚(PNP) 铜绿假单胞菌 生物降解 固定化 

分 类 号：X172[环境科学与工程—环境科学]