重组Ag43-CD154嵌合蛋白诱导抗肝纤维化免疫反应  

Induction of immune response against hepatic fibrosis by recombinant protein Ag43-CD154

作　　者：王才春[1] 陈少兴[1] 黄风迎[2] 林映莹[2] 郑少江[3] 黎岳南[3] 

机构地区：[1]海南医学院附属医院内科,海南海口571101 [2]海南医学院海南省热带病重点实验室,海南海口571101 [3]海南医学院病理科,海南海口571101

出　　处：《海南医学院学报》2012年第5期582-585,共4页Journal of Hainan Medical University

基　　金：海南省自然科学基金项目(808235)~~

摘　　要：目的:了解基因工程技术构建制备获得的CD154-Ag43重组嵌合蛋白是否能够诱导产生抗自身抗CD154抗体的免疫反应。方法:将CD154-Ag43重组蛋白作为模式疫苗免疫刀豆素蛋白诱导肝纤维化模型小鼠,用免疫印迹和酶联免疫斑点试验了解是否能够诱导产生抗CD154抗体,用HE染色观察肝脏纤维化状态,用Masson trichrome和硝酸银染色观察肝脏组织胶原蛋白和网状纤维。结果:免疫印迹发现CD154-Ag43免疫的小鼠可以有效产生抗自身CD154抗体,酶联免疫斑点试验发现脾脏中有特异分泌抗CD154抗体的B淋巴细胞。肝脏组织HE染色发现CD154-Ag43免疫的肝纤维化模型小鼠肝脏纤维化明显轻于对照组,并且肝脏胶原蛋白和网状纤维也明显少于对照组。结论:基因工程技术构建制备获得的CD154-Ag43嵌合蛋白有可能是一种防治肝纤维化的有效模式疫苗。Objective： To investigate whether chimeric recombinant protein Ag43-CD154 can induce immune response against hepatic fibrosis. Methods： Isolated recombinant protein Ag43-CD154 was used as vaccine in murine Con A-induced hepatic fibrosis model. Autoantibody against CD154 in serum and B lymphocytes in spleen were detected by Western blot and ELISPOT assay. HE, Masson trichrome and silver stains were used to observe the state of hepatic fibrosis, collagen and reticular fibre in liver tissues, respectively. Results： Autoantibody against self-CD154 and B lymphocytes secreted autoantibody against self-CD154 were found in serum and spleen, respectively. Significant attenuation of hepatic fibrosis, collagen and reticular fibre were observed in hepatic tissues in the mice injected with recombinant protein Ag43- CD154. Conclusion： Recombinant protein Ag43-CD154 is a potential effective model vaccine for treatment of hepatic fibrosis.

关 键 词：肝纤维化 细菌表面抗原43 CD154 重组蛋白 疫苗 

分 类 号：R575.5[医药卫生—消化系统]