枸杞菊花散对老年大鼠视网膜各层细胞的影响  

Effect of Gouqi Juhua(Lycium Chrysanthemum) Powder on each layer cells of retina of aged rats

作　　者：燕宪涛[1] 路新国[1] 章海风[1] 丁连翠[1] 吴志嵩[1] 

机构地区：[1]扬州大学旅游烹饪学院,扬州225127

出　　处：《中国中医眼科杂志》2012年第2期80-82,共3页China Journal of Chinese Ophthalmology

基　　金：扬州大学大学生科技创新基金(No.B11148)

摘　　要：目的观察枸杞菊花散对老年SD大鼠视网膜各层细胞的影响,探讨其延缓老年性视力衰退的可能机制。方法以18～22月龄的SD大鼠作为老年大鼠模型,分为老年空白对照组,枸杞菊花散低、中、高剂量组,每组10只;2～3月龄大鼠10只,作为青年空白对照组。枸杞菊花散各组予枸杞菊花散灌胃,对照组予生理盐水灌胃。用药35 d后制作视网膜组织病理切片,HE染色,分层计数各层细胞数目。结果枸杞菊花散中、高剂量组大鼠的视网膜神经节细胞层、外核层细胞数,较老年空白对照组升高,差异有统计学意义(P<0.01)。各组间内核层细胞数差异无统计学意义(P>0.05)。结论枸杞菊花散可以延缓视网膜神经节细胞层及外核层细胞衰减,这可能是其延缓大鼠老年性视力衰退的作用机制。OBJECTIVE To investigate the effect of Gouqi Juhua（Lycium Chrysanthemum） Powder on each layer cells of retina of aged SD rats,and to study the possible mechanism for protection of vision.METHODS Eighteen to twenty-two month old SD rats were divided randomly into 4 groups： aged group,low,medium and high dose aged control group with 10 rats in each group.The youth blank group was grouped by 10 SD rats whose age were between 2~3 months.Medlar chrysanthemum powder groups were given medicines via intragastric administration for 35 days,while the control group was given normal saline.Pathological section and hematoxylin and eosin（HE） staining were used to count cells in each layer of retina.RESULTS Compared with the old blank group,the number of retinal ganglion cell（RGC） and outer nuclear layer（ONL） cells of youth blank group and aged control group with low,medium,high dose were significantly increased（P0.01）;The number of inner nuclear layer（INL） cells were not statistically different between each group（P0.05）.CONCLUSIONS Lycium Chrysanthemum Powder could delay the decay of RGC layer and ON layer cells,which might be the mechanism of the progression of age-related vision decline of SD rats.

关 键 词：枸杞菊花散 老年性视力衰退 视网膜 细胞计数 

分 类 号：R285.5[医药卫生—中药学]