TGF-β1和Smad2在胃癌中的表达及意义  

Expression of TGF-β1 and Smad2 in human gastric cancer

作　　者：沈卫东[1] 刘鹏飞[1] 刘兵团[1] 项斌[1] 张伟[1] 

机构地区：[1]东南大学医学院附属江阴医院消化内科,江苏省江阴市214400

出　　处：《世界华人消化杂志》2012年第14期1238-1241,共4页World Chinese Journal of Digestology

摘　　要：目的:检测TGF-β1和Smad2信号在胃癌中的表达,探讨TGF-β1和Smad2在胃癌发生、发展中的作用.方法:采用免疫蛋白印迹(Westernblot)和实时定量PCR检测45例胃癌组织及相应正常组织TGF-β1、Smad2蛋白及mRNA的表达,比较正常对照、癌组织中上述二者表达的差异,并进行统计学分析.结果:TGF-β1在胃癌组织中阳性表达率为77.8%(35/45),在正常组织中为33.3%(15/45),二者比较差异显著(P<0.05);Smd2蛋白在胃癌组织中阳性表达率为73.3%(33/45),在正常组织中为26.7%(12/45),二者比较有显著差异(P<0.05);TGF-β1、Smad2mRNA在胃组织中阳性表达率分别为88.9%(40/45)、84.4%(38/45),均显著高于正常组织(P<0.05).胃癌组织中TGF-β1、Smad2蛋白的表达与病理分级、浸润深度、淋巴结转移、脉管侵犯相关(P<0.05).结论:TGF-β1和Smad2可能在胃癌的发生、发展中发挥作用,并与胃癌的侵袭、转移相关.AIM：To investigate the correlation of the expression of transforming growth factor-1 （TGF-β1） and mothers against decapentaplegic homolog 2 （Smad2） with the carcinogenesis and tumor progression of human gastric cancer.METHODS：Western blot and real-time PCR were used to detect the expression of TGF-β1 and Smad2 protein and mRNA in 45 cases of gastric cancer and matched normal gastric mucosal tissues.RESULTS：The positive rates of TGF-β1 and Smad2 protein expression were significantly higher in gastric cancer than in normal gastric mucosa （77.8% vs 33.3%,73.3% vs 26.7%,both P 0.05）.The positive rates of TGF-β1 and Smad2 mRNA expression were also significantly higher in gastric cancer than in normal tissue （88.9% vs 35.6%,84.4% vs 31.1%,both P 0.05）.The protein expression of TGF-β1 and Smad2 was correlated with pathological grade,lesion depth,lymph node metastasis and vessel invasion.CONCLUSION：Overexpression of TGF-β1 and Smad2 may play an important role in the development and progression of gastric cancer.

关 键 词：转化生长因子-1 细胞信号转导分子-2 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]