异氟醚通过激活Ryanodine受体对ReNcell CX人神经干细胞损伤和存活发挥双向调节作用  

Dual effects of isoflurane on damage and survival in human neural stem cells via activation of Ryanodine receptor

作　　者：赵序利[1,2] 刘艳华 罗剑刚[2] 王珺楠[2] 郑宏[1] 傅志俭[1,2] 

机构地区：[1]新疆医科大学第一附属医院麻醉科,新疆乌鲁木齐830054 [2]山东大学附属省立医院疼痛科,山东济南250021 [3]山东省巨野县人民医院妇产科,山东巨野274900

出　　处：《中国医药科学》2012年第9期11-13,共3页China Medicine And Pharmacy

基　　金：山东省自然科学基金资助项目(ZR2009CQ016)

摘　　要：目的研究Ryanodine受体在吸入性麻醉剂异氟醚对ReNcell CX人神经干细胞损伤和存活的影响中的作用。方法将培养的ReNcell CX人神经干细胞应用2.6%异氟醚分别处理1、4、8、12 h后,测量LDH和MTT,研究异氟醚对ReNcell CX人神经干细胞存活的时间效应;在异氟醚处理前加入Ryanodine受体特异性阻滞剂100 nM丹曲林,再测量LDH和MTT,研究阻断Ryanodine受体能否消除异氟醚对ReNcell CX人神经干细胞存活的影响。结果 2.6%异氟醚处理1 h,LDH低于对照组,MTT高于对照组(P<0.05),说明促进ReNcell CX人神经干细胞存活;2.6%异氟醚处理12 h,LDH高于对照组,MTT低于对照组(P<0.05),说明抑制ReNcell CX人神经干细胞存活;加入Ryanodine受体阻滞剂丹曲林后,再用2.6%异氟醚处理12 h,LDH和MTT与对照组差异无统计学意义(P>0.05),说明异氟醚通过激活Ryanodine受体影响ReNcell CX人神经干细胞存活。结论异氟醚对ReNcell CX人神经干细胞损伤和存活具有双向作用,这种作用通过内质网膜上Ryanodine受体介导。Objective To investigate the influences of isoflurane on cell damage and survival of ReNcell CX human neural stem cells,in turn to assess the relationship between ryanodine receptor and ReNcell CX human neural stem cell damage and survival. Methods The ReNcell CX human neural stem cells cultured in vitro were exposed to different duration of isoflurane （1 h,4 h,8 h and 12 h respectively） with or without dantrolene,a specific ryanodine receptor antagonist. Then,LDH and MTT were analyzed to assess the effects of isoflurane and dantrolene on ReNcell CX human neural stem cell damage and survival. Results Isoflurane induced ReNcell CX human neural stem cells damage and inhibition on ReNcell CX human neural stem cell survival in a time dependent manner. Short time exposure to 2.6% isofturane（1 h） promoted ReNcell CX human neural stem cell survival and inhibited ReNcell CX human neural stem cells damage. While Prolonged 2.6% isoflurane exposure inhibited ReNcell CX human neural stem cell survival and induced ReNcell cX human neural stem cells damage. This dual effects of isoflurane on ReNcell CX human neural stem cells can be antagonized by ryanodine antagonist dantrolene. Conclusion Isoflurane mediated dual effects on ReNcell CX human neural stem cell damage and survival via activation of ryanodine receptors.

关 键 词：钙离子 RYANODINE受体 神经保护 神经毒性 

分 类 号：R741[医药卫生—神经病学与精神病学]