MHV-3诱导的小鼠病毒性肝炎模型肾脏中IL-17A表达的实验研究  

Expression of IL-17A in kidney of MHV-3 induced murine viral hepatitis model

作　　者：陆玉蕾[1] 林有智[2] 朱琳[1] 王晓晶[1] 严伟明[1] 宁琴[1] 

机构地区：[1]华中科技大学附属同济医院感染性疾病研究所感染病科 [2]华中科技大学附属同济医院肝脏外科实验室,湖北武汉430030

出　　处：《中国医药科学》2012年第9期43-44,共2页China Medicine And Pharmacy

摘　　要：目的研究3型鼠肝炎病毒诱导的小鼠病毒性肝炎模型中肾脏组织细胞因子IL-17A的表达及其意义。方法利用MHV-3病毒诱导小鼠病毒性肝炎模型,采用免疫组化方法检测肾脏组织中IL-17A的表达;采用逆转录-聚合酶链反应法检测肾脏组织中IL-17A mRNA水平。结果肝炎模型小鼠肾脏组织中表达IL-17A,随着MHV-3感染时间的延长,肾脏组织中IL-17A mRNA水平逐渐增高,在第10天达到高峰。结论肾脏组织中高表达的IL-17A可能参与MHV-3诱导的小鼠病毒性肝炎模型中MHV-3感染引起的肾脏损伤的病理过程。Objective To investigate the expression of IL-17A in the kidney injury induced murine viral hepatitis model. Methods MHV-3 induced murine viral hepatitis model was adopted in the research and the expression of IL-17A in kidney tissue was detected by immunohistochemistry and RT-PCR respectively. Results The expression of IL-17A was detectable in kidney tissue after MHV-3 infection. By the time-course of MHV-3 infection, the level of IL-17A mRNA in kidney were elevated significantly, and reached the highest level after 10 days post MHV-3 infection. Conclusion The increased expression of IL-17A may play an important role in the pathogenesis of renal injury induced by hepatitis viral infection.

关 键 词：MHV-3 病毒性肝炎 IL-17A 

分 类 号：R575.1[医药卫生—消化系统]