脑源性神经营养因子及其受体原肌球蛋白相关激酶B在肝癌组织中的表达以及对肝癌细胞97-H凋亡和侵袭作用的影响  

Expressions of brain-derived neurotrophic factor and its receptor Tropomyosin-related kinase B in hepatocellular carcinoma and their roles in the apoptosis and invasion of hepatocellular cancer 97-H cells

作　　者：郭大伟[1] 刘武[1] 孙文郁[1] 朱磊[1] 张弘彬[1] 侯学忠[2] 姜晓峰[1] 梁健[1] 

机构地区：[1]中国医科大学附属第四医院普外科,沈阳110032 [2]中国人民解放军463医院普外科,沈阳110042

出　　处：《肿瘤》2012年第5期337-341,共5页Tumor

基　　金：辽宁省教育厅2009年度高等学校科研资助项目(编号:2009A729)

摘　　要：目的:研究脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)及其受体原肌球蛋白相关激酶B(Tropomyosin-related kinase B,TrkB)在人肝细胞癌(hepatocellular carcinoma,HCC)中的表达情况,并探讨2者在HCC发生、发展中的作用。方法:采用蛋白质印迹法检测BDNF和TrkB蛋白在30例HCC和癌旁组织中的表达情况。采用ELISA法检测BDNF在人HCC细胞系97-H培养液上清中的浓度;FCM和Transwell小室法分别检测抗BDNF抗体或TrkB激酶活性抑制剂K252a对细胞凋亡和侵袭的影响。结果:30例配对组织标本中,BDNF和TrkB在HCC组织中的表达水平高于癌旁组织(P均<0.05)。BDNF在97-H细胞培养液上清中的表达量为(119.08±6.21)pg/mL。抗BDNF抗体或K252a都能有效诱导97-H细胞的凋亡,并抑制细胞的侵袭能力。结论:BDNF/TrkB可能对HCC细胞的存活和侵袭具有重要的支持作用,并促进HCC的发生及发展。Objective：To investigate the expressions of brain-derived neurotrophic factor（BDNF） and its primary receptor tropomyosin-related kinase B（TrkB） in human hepatocellular carcinoma（HCC） tissues,and to evaluate the functions of both the proteins in the oncogenesis and progression of HCC.Methods：The expressions of BDNF and TrkB proteins in HCC tissues and their adjacent normal tissues from 30 patients were detected by Western blotting.The level of secretory BDNF from human HCC 97-H cells in culture supernatant was measured by ELISA.The effects of BDNF neutralizing antibody or Trk tyrosine kinase inhibitor K252a on apoptosis and invasion were detected by flow cytometry（FCM） and Transwell assay,respectively.Results：The expression levels of BDNF and TrkB proteins in HCC tissues from 30 patients were significantly higher than those in the corresponding adjacent normal tissues（P〈0.05）.The concentration of BDNF in culture supernatant of 97-H cells was（119.08±6.21） pg/mL.Both of BDNF neutralizing antibody and K252a could effectively induce the apoptosis and suppress the invasion of 97-H cells.Conclusion：BDNF/TrkB plays an important role in the survival and invasion of HCC cells and can promote the oncogenesis and progression of HCC.

关 键 词：癌 肝细胞 脑源性神经营养因子 原肌球蛋白相关激酶B 肿瘤转移 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]