三七皂苷单体Rg1对低氧高二氧化碳肺动脉平滑肌细胞p38MAPK表达的影响  

Effect of notoginsenoside Rg1 on p38MAPK expression of pulmonary arterial smooth muscle cells exposed to hypoxia hypercapnia

作　　者：唐兰兰[1,2] 郝卯林[1] 黎关龙[1] 王园园[1] 赵珊[1] 刘亚坤[1] 王淑君[1] 王万铁[1] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325035 [2]浙江省中医药大学附属第二医院病理科,杭州310005

出　　处：《中国应用生理学杂志》2012年第3期230-233,共4页Chinese Journal of Applied Physiology

基　　金：浙江省中医药科技计划重点项目(2008ZA017)

摘　　要：目的:研究三七皂苷单体Rg1对低氧高二氧化碳肺动脉平滑肌细胞(PASMCs)p38MAPK表达的影响。方法:分离、纯化SD大鼠PASMCs,实验用2至5代细胞,实验分六组:常氧组(N组),低氧高二氧化碳组(H组),DM-SO对照组(HD组),Rg1干预组(RgL、RgM、RgH组)。采用Western blot检测磷酸化p38MAPK蛋白表达,RT-PCR检测p38MAPK mRNA的表达。结果:Westernblot、RT-PCR结果显示,HD组p-p38MAPK蛋白和p38MAPK mRNA表达明显高于N组(P<0.01),RgL、RgM、RgH组不同程度抑制了p-p38MAPK蛋白和p38MAPK mRNA和的表达(P<0.01),并呈剂量依赖关系。结论:三七皂苷单体Rg1对低氧高二氧化碳条件下PASMCs有保护作用,其机制可能与抑制p38MAPK的表达有关。Objective： To study the effect of Notoginsenoside Rgl on p38 mitogen activated protein kinase （p38MAPK） expression in pulmonary artery smooth muscle cells （PASMCs） cultured in hypoxia hypercapnia. Methods： SD rat PASMCs was primary cultured, the cells of passage 2- 5 were divided into six groups： nonnoxic group （N group）, hypoxia hypercapnia group （H group）, dimethyl sulfoxide （DMSO） control group （HD group）, Rgl treated group （Rg low dose, Rg middle dose and Rg high dose group）. Western blot was used to detect the expression of p-p38MAPK protein, and RT-PCR to determine the expression of p38MAPK mRNA. Results： Western blot and RT-PCR analysis indicated that the expression of p-p38MAPK protein and p-p38MAPK mRNA were significantly higher in HD group than those in N group （ P 〈 0. 01）. Whereas, in Rgl treated groups, the level of p-p38MAPK markedly decreased （P 〈 0.01） in dose-dependent manner. Conclusion： Notoginsenoside Rgl has protective effects on PASMCs under hypoxia hypereapnia condition, which may be related to inhibiting expression of p38MAPK.

关 键 词：低氧高二氧化碳 肺动脉高压 p38㈣通路 三七皂苷单体Rg1 

分 类 号：R363[医药卫生—病理学]