碱性成纤维生长因子基因单核苷酸多态性与广西壮族男性骨质疏松的关系  

Correlation between basic fibroblast growth factor gene single nucleotide polymorphisms and osteoporosis in male of Zhuang nationality in Guangxi Baise

作　　者：林朝文[1] 黄秀峰[1] 王金花[1] 周庆辉[1] 杨园园[1] 韦叶生[2] 韦荣耀[1] 陈秉朴[1] 

机构地区：[1]右江民族医学院人类学研究所 [2]右江民族医学院附属医院检验科,广西百色533000

出　　处：《解剖学报》2012年第3期417-421,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30860117);广西科学研究与技术开发计划资助项目(桂科计字[2008]98号);右江民族医学院攻关资助项目([2008]2号)

摘　　要：目的探讨碱性成纤维生长因子(bFGF)基因5个单核苷酸多态性(SNPs)与广西百色地区壮族男性骨密度(BMD)的关系。方法选取壮族男性147例跟骨骨量减少患者和154例骨量正常对照进行病例对照研究,采用单碱基延伸的单核苷酸多态性分型技术对广西百色地区301例壮族男性的脂联素基因的5个位点(rs308379、rs12644427、rs3789138、rs308442和rs3747676)进行了基因分型,采用法国生产的Osteospace干式超声骨密度仪测量右侧跟骨超声振幅衰减(BUA)。结果 rs308379、rs12644427、rs3789138、rs308442和rs3747676多态性分布均符合Hardy-Weinberg遗传平衡定律(P>0.05)。以5个多态性位点作为自变量的多元Logistic回归显示,仅rs308442多态性与跟骨骨量减少显著相关(OR=1.831,95%CI:1.075～3.116,P=0.026)。结论 bFGF基因第1内含子rs308442多态性与壮族男性BMD可能有一定关联,其中TT型可能对BMD具有一定的保护作用,TA型是BMD降低的危险因素。rs308379、rs12644427、rs3789138及rs3747676多态性与壮族男性BMD无相关性。Objective To investigate the correlation of the basic fibroblast growth factor （bFGF） gene 5 single nucleotide polymorphisms（SNP） and bone mineral density（BMD） in Guangxi Zhuang nationality men. Methods A case-control study was carried out on 147 osteopenia patients and 154 matched controls. Inclusion criteria were at the age of 41 to 78, Zhuang nationality,living in Baise more than 10 years, and no history of taking drugs affecting bone metabolism. Exclusion criteria included secondary osteoporosis, any disease affecting bone mineral density or consanguinity. Genotypes for adiponectin gene 5 locis （rs308379,rs12644427,rs3789138,rs308442 and rs3747676） polymorphism were determined by Multiplex SNaPshot. Broadband ultrasound attenuation （BUA） for the right leg calcaneal was measured by French production of Osteospace dry ultrasound bone densitometer. Results rs308379,rs12644427,rs3789138,rs308442 and rs3747676 polymorphisms were met with Hardy-Weinberg equilibrium （P 〉 0. 05 ）. Five locis genotype frequencies in the normal group and the osteopenia group were met with Hardy-Weinberg equilibrium （ P 〉 O. 05 ）. There was no significant difference in the genotype distributions of five loeis polymorphism between LBM group and control group （P 〉 0. 05 ）. Multivariate Logistic regression analyses revealed only rs308442 polymorphism remained significantly associated with low bone mass（LBM） （P 〈 0. 05）. The subjects with the combined TA genotype had higher risk of LBM compared with those with the TT genotype （ OR = 1. 831,95% CI： 1. 075-3.116, P = 0. 026 ）. Polymorphism of rs308442 was independently correlated with LBM at the calcaneal in Baise Zhuang femail population. Conclusion bFGF gene intron 1 rs308442 polymorphism and BMD in Baise Prefecture Zhuang men have some relevance. The presence of the T allele A may dominantly increase the risk of osteopenia. TT genotype has a protective effect of BMD. The data also suggest that the rs308379, rs12644427, rs3789138 and rs3

关 键 词：壮族 单核苷酸多态性 碱性成纤维生长因子 骨质疏松症 遗传多态性 多重单碱基延伸SNP分 型技术 男性 

分 类 号：R592[医药卫生—老年医学]