古尼拟青霉和蝙蝠蛾拟青霉发酵菌丝体挥发性成分的分析与比较  

Analysis and comparison of volatile components of mycelia of Paecilomyces gunnii and Paecilomyces hepiali

作　　者：于士军[1] 张莹[1] 蒋岚[1] 包佳源[1] 李春如[1] 樊美珍[1] 

机构地区：[1]安徽农业大学安徽省微生物防治重点实验室,安徽合肥230036

出　　处：《菌物学报》2012年第3期374-381,共8页Mycosystema

基　　金：国家高技术研究发展计划资助项目(863计划)(No.2007AA021506)

摘　　要：对蝙蝠蛾拟青霉Paecilomyces hepiali和古尼拟青霉P.gunnii菌丝体的挥发性成分进行了研究,采用同时蒸馏萃取法分别提取两种菌丝体中的挥发性成分,经GC-MS联用仪对挥发性成分进行分离鉴定,分别分析出25种和32种挥发性成分。在蝙蝠蛾拟青霉菌丝体挥发性成分中2,6-二叔丁基对甲酚和1,5-二氢-1-甲基-2H-吡咯-2-酮相对含量较高,分别占65.78%和12.77%;在古尼拟青霉菌丝体中挥发性成分2,6-二叔丁基对甲酚和(E,E)-2,4-癸二烯醛相对含量较高,分别占62.11%和12.32%。两种菌丝粉共有8种共有成分,分别占蝙蝠蛾拟青霉挥发性成分的71.58%,古尼拟青霉挥发性成分的69.67%;由此可见蝙蝠蛾拟青霉和古尼拟青霉菌丝体主要挥发性物质的成分是相同的。通过对蝙蝠蛾拟青霉和古尼拟青霉挥发性成分的研究,为我们进一步了解这两种真菌菌丝体的药理作用提供了实验依据。The volatile components of mycelia of Paecilomyces hepiaB and Paecilomyces gunnii were extracted by simultaneous distillation extraction （SDE） and analyzed by gas chromatography-mass spectrometry （GC-MS）. The results indicated that there were 25 and 32 volatile components in mycelia of P. hepiali and P. gunnii, respectively. Butylated hydroxytoluene and 2H-pyrrol-2-one,l,5-dihydro-l-methyl are the two major volatile components in the mycelia of P. hepiali, accounting to 65.78% and 12.77%, respectively. Butylated hydroxytoluene and （E,E）-2,4-decadienal are the two major volatile components in the mycelia ofP. gunnii, accounting to 62.11% and 12.32%, respectively. Eight components are in common in liquid submerged fermentation mycelia of the two fungi and make up 71.58% and 69.67% of the total volatile components, respectively, indicating that the major volatile components in the two experimental mycelia are similar. Theanalysis of volatile components provided a better understanding of the pharmaceutical activities of the mycelia of P. hepiali and P. gunnii.

关 键 词：蝙蝠蛾拟青霉 古尼拟青霉 挥发性成分 气质联用仪 

分 类 号：S567.35[农业科学—中草药栽培]