人肺腺癌细胞系Ch-Huang-1的建立和生物学特性研究  

Establishment and Characterization of A Human Lung Adenocarcinoma Cell Line,Ch-Huang-1

作　　者：李白翎[1] 钟铿[1] 金磊[1] 袁扬[1] 龚德军[1] 刘晓红[1] 黄盛东[1] 

机构地区：[1]第二军医大学附属长海医院胸心外科,上海200433

出　　处：《中国肺癌杂志》2012年第5期267-270,共4页Chinese Journal of Lung Cancer

基　　金：上海市科委医学引导基金(No.114119a9300)资助~~

摘　　要：背景与目的肺癌是严重威胁人类生存和发展的恶性疾病之一,本研究旨在建立人肺腺癌细胞系,探讨其生物学特性,为进一步明确肺癌的发生机理,提供一个技术平台。方法取人肺腺癌新鲜手术肿瘤标本,经组织块体外培养并克隆建系,命名为Ch-Huang-1。采用光镜、细胞生长曲线、染色体核型分析、克隆形成率及免疫缺陷小鼠成瘤实验对细胞系生物学特性进行分析。结果细胞系及移植瘤标本证实具有腺癌恶性细胞特征。生长曲线呈S型,细胞群体倍增时间为36 h。分裂指数曲线为倒V字型,培养第30小时处于分裂期的细胞最多。克隆形成率为0.803%±0.078%。观察到肿瘤细胞染色体数目为亚三倍体,有明显的染色体异常。裸鼠皮下异种移植形成移植瘤。结论根据细胞系特征显示该细胞系是一新建的肺腺癌细胞系。Background and objective Lung cancer is a major worldwide health problem.The aim of this study is to establish a novel Chinese human lung adenocarcinoma cell line and examine its biological characteristics.Methods Lung adenocarcinoma specimens were freshly resected during surgery.The tissues were incubated in vitro and the cell line was named Ch-Huang-1.The biological characteristics of the cells were investigated by light microscopy,chromosome analysis,and transplantation experiment.Results Light microscopy revealed that cells from the primary tumor,Ch-Huang-1 cell line,and transplanted tumor possessed the characteristics of a malignant glandular epithelial tumor.The cell growth curve,doubling time,and mitotic index were also observed in vitro.Nuclear chromosome analysis revealed that the tumor was a subtriploid with a mode of 35-44 per cell.Tumor nodes were observed under the skin of nude mice by heterogenic transplantation.Conclusion The characteristics of the established cell line suggest that it is a newly established human adenocarcinoma cell line.

关 键 词：肺肿瘤 细胞系 生物学特性 

分 类 号：R734.2[医药卫生—肿瘤]