RT-PCR及激光共聚焦检测人绒毛和绒癌细胞中AE2的表达  

The detection of expression of AE2 in human trophocyte and JAR cell line by RT-PCR and immunofluoreseence staining confocal laser imaging

作　　者：王思义 张建华[2] 张华[2] 

机构地区：[1]成都市妇女儿童中心医院妇产科,四川成都610091 [2]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《四川医学》2012年第4期586-588,共3页Sichuan Medical Journal

摘　　要：目的探讨在人正常绒毛细胞和绒癌JAR细胞中阴离子交换蛋白2(AE2,Anion Exchanger 2)的表达差异及意义。方法以RT-PCR技术及免疫荧光激光共聚焦技术检测人正常绒毛滋养细胞和绒毛膜癌细胞株JAR细胞膜上AE2的表达。结果①在人绒毛滋养细胞与人绒癌JAR细胞株中,均检测到AE2mRNA的表达。绒癌组AE2的OD值为(0.85±0.21);正常组AE2的OD值分别为(0.19±0.01)。绒癌组AE2的OD值明显高于正常组(P<0.05)。②免疫荧光激光共聚焦实验证实AE2在绒癌组呈红色阳性表达,在正常组中未见红光,当加强激发强度后,仍可探及AE2在正常组的表达。结论 AE2在绒癌中表达异常升高,可能引起滋养细胞内水电解质平衡紊乱,导致绒癌发生。Objective To discuss differential expression of anion exchanger 2 in cultured normal human trophocyte and JAR cell line,and its significance in the development of cancer.Methods RT-PCR and immunofluoreseence staining confocal laser imaging were used to detected the expression of AE2 in cultured normal human trophocyte and JAR cell.Results ① We could detect the expression of anion exchanger 2 in both cultured normal human trophocyte and JAR cell line.Choriocarcinoma group AE2,respectively,for the OD value（0.85 ± 0.21）,normal group,AE2,respectively,for the OD value（0.19 ± 0.01）.The OD value of AE2 in choriocarcinoma group was significantly higher than that in the normal group（P0.05）.②By immunofluoreseence staining confocal laser imaging,it was revealed that,in the same condition and excitation intensity,AE2 in the JAR ehoriocareinoma cell lines showed positive expression of the red,while in norm al trophoblast cells,no red light,but when the excitation intensity was strengthened,it may still explore AE2 in normal human villous trophoblast cell expression.Conclusion The overexpression of AE2 may lead to the imbalance of water and electrolyte in trophocyte,And it may be involved in the happen of choriocarcinoma.

关 键 词：AE2 绒癌 JAR细胞株 

分 类 号：R737.33[医药卫生—肿瘤]