小鼠骨髓间充质干细胞移植后在炎症性肠病模型的定位  

Localization of transplanted bone marrow mesenchymal stem cells in mouse inflammatory bowel disease model

作　　者：陈倩倩[1] 万军[1] 阎丽[1] 王卫华[1] 王昌正[1] 石卉[1] 苏斌斌[1] 曾庆环[1] 杜海涛[1] 

机构地区：[1]解放军总医院南楼消化科,北京100853

出　　处：《军医进修学院学报》2012年第6期638-641,共4页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金项目(81050027)~~

摘　　要：目的探讨小鼠骨髓间充质干细胞(BMSCs)移植治疗小鼠炎症性肠病(IBD)模型后其在结肠的定位情况。方法将骨片培养法培养的雄性BALB/C小鼠BMSCs,用羧基荧光素二乙酸盐琥珀酰亚胺酯(CFDA SE)进行荧光标记后经尾静脉注射于雌性大鼠IBD模型体内,并设立对照组。分别于移植后第2、5、9天后取远端结肠组织,一部分制成冰冻切片于荧光显微镜下观察结肠荧光分布情况,另一部分经PCR检测Y染色体的性别决定区段(SRY)基因作为标志,以确定BMSCs的肠道定位情况。结果 BMSCs生长迅速、纯度高,可诱导成为脂肪细胞及骨细胞;2,4,6-三硝基苯磺酸(TNBS)造成的IBD模型部分肠段缩窄,溃疡形成,黏膜及黏膜下层有大量中性粒细胞、淋巴细胞、巨噬细胞及纤维细胞浸润;荧光显微镜下可见移植BMSCs的模型组结肠组织存在荧光;SRY检测显示TNBS-MSCs移植组及雄性小鼠对照组均能检测到SRY基因。结论移植后的BMSCs能在IBD模型的损伤肠道组织中定位。Objective To localize the transplanted bone marrow mesenchymal stem cells（BMSCs） in colon of a mouse inflammatory bowel disease（IBD）model.Methods BMSCs from BALB/C male mice,cultured in bone slice culture in vitro,were transplanted into a BALB/C female mouse IBD model through the caudal vein,after marked with fluorescein CFDA SE.A control group was established.Distal colon tissue was taken on days 2,5,and 9 after transplantation.The tissue was frozen and cut into sections to observe the fluorescence distribution in BMSCs and the location of BMSCs using the sex determining gene（SRY） on Y chromosome as a marker as detected by PCR.Results Adipocytes and osteoblasts were induced by the rapid proliferation and high purity of BMSCs.Some segments of colon in IBD model became narrow with ulceration due to TNBS.Fluorescence was found in colon tissue from IBD model under fluorescence microscope.The SRY gene was detected both in TNBS-MSCs transplantation group and in control group.Conclusion Transplanted BMSCs can be localized in injured colon tissue of IBD model.

关 键 词：骨髓间充质干细胞 移植 炎症性肠病 2 4 6-三硝基苯磺酸 生物学标记 

分 类 号：R574[医药卫生—消化系统]