环氧合酶-2在低分子肝素干预大鼠酒精性肝病中的作用机制研究  

Effect of cyclooxygenase-2 in alcoholic liver disease rats intervened with low molecular weight heparin

作　　者：阎春英[1] 石军[1] 杨艳[1] 郝菁华[1] 任万华[1] 张捷[2] 

机构地区：[1]山东大学附属省立医院肝病中心,山东济南250021 [2]山东大学附属省立医院中心实验室,山东济南250021

出　　处：《胃肠病学和肝病学杂志》2012年第5期466-469,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：山东省优秀博士基金计划课题(2005BS03207)

摘　　要：目的研究低分子肝素干预大鼠酒精性肝病模型的作用机制。方法 56%(V/V)的白酒平均以8 g/kg的剂量2次/d灌胃10周,初步制备大鼠酒精性肝病模型,成模后部分大鼠低分子肝素(100 IU/kg)皮下注射进行干预,4周后观察低分子肝素对大鼠的血清生物化学指标、肝组织中丙二醛及环氧化酶-2的影响。结果与模型组比较,低分子肝素治疗组大鼠血清转氨酶、血脂及丙二醛含量明显下降(P<0.05);光学显微镜观察HE染色见脂肪变性、炎症反应程度明显减轻(P<0.01),免疫组织化学染色及逆转录聚合酶链反应显示环氧化酶-2的表达均明显降低(P<0.05)。结论低分子肝素通过改善脂肪代谢、抑制氧化应激及降低环氧化酶-2的表达而对酒精性肝病大鼠起到一定的保护治疗作用。Objective To study the effect of low molecular weight heparin on alcohol-fed rats. Methods The mod- el of alcoholic liver disease rats was made by alcohol gavage （infused with 56% of ethanol twice a day with a dose of 8 g/kg body weight for 10 weeks） , and then parts were treated with low molecular weight heparin （ 100 IU/kg）. After 4 weeks, the effect of low molecular weight heparin on serum biochemical index, malondialdehyde and cyclooxygenase-2 in liver tissue was observed. Results Compared with model group, the levels of transaminases, lipids and malondialde- hyde significantly decreased in low molecular weight heparin therapy group （P 〈 0.05 ）. Under the observation of optical microscope, the degree of liver steatosis, inflammation reaction were significantly reduced （P 〈 0.01 ）. The levels of cy- clooxygenase-2 in liver tissue were significantly lowered by immunohistochemical staining and semiquantitative reverse transcription-polymerase chain reaction. Conclusion Low molecular weight heparin has therapeutic effect on alcohol- induced liver disease rats by improving fat metabolism, reducing oxidative stress and decreasing the expression of cy- clooxygenase-2.

关 键 词：酒精性肝病 低分子肝素 氧化应激 环氧化酶-2 

分 类 号：R575[医药卫生—消化系统]