腺苷受体A_3在特发性炎症性肌病中的表达  

A_3 adenosine receptor expression in idopathic inflammatory myopathies

作　　者：遇晓[1] 于丕先 赵丹丹[3] 刘付臣[3] 焉传祝[3] 

机构地区：[1]山东大学齐鲁医院风湿科,济南250012 [2]山东大学附属济南市中心医院神经内科,济南250012 [3]山东大学齐鲁医院神经内科神经肌肉病实验室,济南250012

出　　处：《山东大学学报（医学版）》2012年第6期105-109,113,共6页Journal of Shandong University:Health Sciences

摘　　要：目的探讨腺苷受体A3(A3AR)在特发性炎症性肌病(IIMs)发病机制中的作用。方法收集IIMs患者骨骼肌活检组织标本30例,其中皮肌炎(DM)患者15例、多发性肌炎(PM)患者11例、包涵体肌炎(IBM)患者4例;正常对照标本12例。在冰冻切片上行组织化学染色和A3AR抗体免疫荧光染色。结果 A3AR抗体免疫荧光染色阳性见于骨骼肌2型肌纤维胞浆,1型肌纤维胞浆呈阴性;与正常对照比较,30例IIMs患者中22例2型肌纤维胞浆荧光增强。肌纤维膜荧光染色在正常对照呈阴性,而19例IIMs患者呈阳性。血管平滑肌和部分坏死肌纤维A3AR抗体免疫荧光染色呈强阳性。结论人骨骼肌2型肌纤维表达A3AR;特发性炎症性肌病患者A3AR表达增加,可能对肌纤维的修复和凋亡起双向调节作用。Objective To investigate the effects of A3 adenosine receptor（ A3 AR）expression in the pathogenesis of ido- pathic inflammatory myopathies（IIMs）. Methods Muscle tissues from 30 cases of IIMs including 15 cases of derma- tomyositis （ DM）, 11 cases of polymyositis （ PM）, 4 cases of inclusion body myositis （IBM） and 12 cases of normal controls were collected. Histochemical staining and immunofluorescent staining of the A3 AR antibody were used to de- tect the existence of A3AR in the muscle biopsies. Results The cytosolic immunofluorescent staining of A3AR anti- body was positive in the type II muscle fibers, however, the staining was absent in the type I muscle fibers. 22 cases among the 30 cases of IIMs displayed a more intense cytosolic staining compared with that of the controls. Cell mem- brane fluorescent staining was positive in 19 cases of the IIMs, but it was negative in the controls. The highest intensity of the irnmunofluorescent staining for the A3 AR antibody was localized in vascular smooth muscles and some necrotic fi- bers. Conclusions The A3AR expresses in human type 11 skeletal muscle fibers. Expression of A3AR increases in idopathic inflammatory myopathies （IIMs） patients, and it may have a bidirectional regulation in the repairing and ap- optosis of muscle fibers.

关 键 词：受体 嘌呤能P1 肌炎 活组织检查 肌肉 

分 类 号：R392.31[医药卫生—免疫学]