男性泌尿生殖道感染非淋病奈瑟菌的毒力相关基因检测  

Virulence-associated Gene Determination of Non-gonococcal Neisseria Strains Isolated from Male Genitourinary Tracts

作　　者：王涛[1] 王和[1] 佘晓玲[1] 叶长芸[2] 

机构地区：[1]贵阳医学院微生物学教研室,贵州贵阳550004 [2]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《中国皮肤性病学杂志》2012年第6期477-479,共3页The Chinese Journal of Dermatovenereology

基　　金：贵州省科技基金(SY[2010]3045);传染病预防控制国家重点实验室自主研究开放课题基金(2011SKLID302);贵州省卫生厅科技基金(gzwkj2009-1-028)

摘　　要：目的检测男性泌尿生殖道分离的非淋病奈瑟菌毒力相关基因,探讨非淋病奈瑟菌的致病机理。方法采用PCR扩增和核苷酸序列分析技术,检测从男性泌尿生殖道感染患者生殖道分离的6个菌种12株非淋病奈瑟菌的毒力相关基因orf1,nspA,PIA,PIB,opa,iga,pilE。结果 12个分离菌株中8株(检出率66.67%)检出毒力相关基因,其中orf1的阳性率为25.00%(3/12),opa为25.00%(3/12),PIB为16.67%(2/12),nspA为8.33%(1/12),各菌株均未检出PIA,pilE与iga。结论感染男性泌尿生殖道的部分非淋病奈瑟菌具有淋病奈瑟菌的毒力相关基因。但这些毒力相关基因并不是非淋病奈瑟菌引起男性生殖道感染的唯一致病因素,非淋病奈瑟菌的男性生殖道致病性可能与其黏膜寄生性等生物学特性有关。Objective To detect virulence-associated genes of non-gonococcal Neisseria isolated from male genitourinary tracts and survey pathogenesis of non-gonococci Neisseria species.Methods By PCR amplification and nucleotide sequencing,the virulence-associated genes of N.gonorrhoeae,including orf1,nspA,PIA,PIB,opa,iga,pilE genes of 12 strains non-gonococci Neisseria,which were distributed in 6 different species of Neisseria,isolated from the patients with male genitourinary tract infection were detected.Results In the 12 strains of non-gonococcal Neisseria species,8 strains showed positive in the reaction of virulence-associated genes,in which 25.00%（3/12） strains were orf1or opa,16.67%（2/12）strains were PIB and 8.33%（1/12）strains were nspA.The genes of PIA,pilE and iga were not found in the species of non-gonococcal Neisseria.Conclusion The virulence-associated genes orf1,nspA,PIB and opa of N.gonorrhoeae can be detected in the species of non-gonococcal Neisseriae.While the above genes are not the only pathogenic factors for the non-gonococci Neisseriae causing male genitourinary tract infection,and the pathogenicity of the non-gonococci Neisseriae infected male genitourinary tract are responsible for their mucosal parasitic properties and other biological properties.

关 键 词：奈瑟菌属 泌尿生殖道感染 毒力基因 基因检测 

分 类 号：R691.3[医药卫生—泌尿科学]