腹侧黄斑小鼠的部分特征及其突变基因的染色体定位  

Phenotype analysis and mutant gene location of ventral yellow mouse(VY^(Slac))

作　　者：施美莲 徐平 殷筱舒[2] 杨伟伟[2] 顾美儿[2] 俞利平[2] 刘桂杰[2] 吴宝金[2] 

机构地区：[1]上海斯莱克实验动物有限责任公司,上海201615 [2]杭州师范大学实验动物中心,浙江杭州310036

出　　处：《Zoological Research》2012年第3期290-297,共8页动物学研究（英文）

基　　金：上海市科学技术委员会科研计划项目(10140902700);国家自然科学基金项目(31071092)

摘　　要：腹侧黄斑小鼠(VYSlac)是在B6小鼠繁殖生产过程中被发现、分离的突变系小鼠,呈单基因显性遗传,其头颈及躯干的腹侧为黄色。VYSlac腹部表皮多巴(+)黑色素细胞及毛囊内黑色素较其背景品系B6少;腹部毛发颜色浅黄、多数无黑色素沉积,但结构正常。通过微卫星标记与48只F2小鼠(VYSlacD2F1回交D2)的连锁分析发现,突变基因与D2Mit229间的LOD值为5.79,确定连锁。随后,在突变基因附近反复多次筛选新的微卫星或SNP标记,通过对196例F2小鼠的多次连锁分析,将突变基因所在区域缩小到rs13476833(距着丝粒149804749bp)与rs27310903(距着丝粒155060764bp)间约5256015bp的范围内。The ventri-yellow pigmentation mouse （temporarily named VY^Slac） arose spontaneously in the C57BL/6J inbred mouse strain, found and bred by Shanghai SLAC Laboratory Animal Co., Ltd. VY^Slac presented a special phenotype marked by yellow coat on the ventral surface of neck and trunk that was without melanin deposition but maintained a normal structure. The number of melanocytes in epidermis and melanin in hair follicle of the abdominal skin of the mutant mouse were less than that of their background strain, while there was no significant difference between the dorsal skins of the two strains. This mutant phenotype was inherited as single-gene dominant inheritance, confirmed by genetic experiment, and there was no significant difference between VY^Slac and B6 for other biological parameters such as weight, anatomic and histological structures of major organs and blood physiology. When the linkage relationship between the genomic DNA samples of F2 48 mice （VY^SlacD2F1×D2） and mutant phenotype were evaluated, the mutant gene was confirmed on chromosome 2 near D2Mit229. New microsatellite and SNP markers were selected to amplify genomic DNA samples of 196 F2 mice and the mutant gene was narrowed down to 5.3 Mb region between rs13476833 and rs27310903 on chromosome 2. The preliminary results of our phenotype analysis and gene location provides a solid basis for further identification of this mutant gene.

关 键 词：腹部黄斑小鼠 表型分析 基因定位 

分 类 号：Q953[生物学—动物学]