ERK信号传导通路在β-榄香烯诱导肾癌细胞凋亡中作用的研究  

Role of ERK signaling pathway in β-elemene-induced apoptosis in human renal cell carcinoma cells

作　　者：詹运洪[1] 孙明[1] 刘静[2] 刘云鹏[2] 曲秀娟[2] 侯科佐[2] 吴斌[1] 

机构地区：[1]中国医科大学附属盛京医院泌尿外科,辽宁沈阳110004 [2]中国医科大学附属第一医院肿瘤内科,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2012年第5期321-323,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30770993)

摘　　要：目的:探讨ERK信号传导通路在β-榄香烯诱导肾癌细胞凋亡中的作用。方法:以人肾透明细胞癌786-0细胞为研究对象,四甲基偶氮唑蓝(MTT)比色法测定细胞活力;流式细胞术碘化丙啶(PI)染色检测细胞凋亡;蛋白印迹法检测细胞内磷酸化ERK及总ERK的表达水平。为研究ERK通路活性对β-榄香烯抗肿瘤作用的影响,将实验分为空白对照组,β-榄香烯单药组(160μg/mL)、ERK抑制剂组(PD98059,20μmol/L)和联合用药组(PD98059+β-榄香烯)。结果:40、80、160及320μg/mL的β-榄香烯作用于786-0细胞24h,细胞生存率逐渐下降,分别为(83.20±4.63)%、(74.29±3.50)%、(49.75±3.08)%和(16.99±4.73)%,相邻浓度间P均<0.05。随着浓度的增加,细胞凋亡率逐渐增加。进一步研究发现,β-榄香烯可明显抑制细胞内磷酸化ERK的水平。同β-榄香烯单药组相比,联合用药组的细胞活力明显降低,差异有统计学意义〔(52.73±6.36)%vs(39.24±3.24)%〕,P<0.05。结论:β-榄香烯可能通过抑制ERK通路活性发挥对肾癌细胞的抗肿瘤作用。OBJECTIVE.. To investigate the role of ERK signaling pathway in 9-elemene-induced apoptosis in human renal cell carcinoma（RCC） cells. METHODS： Human renal cell carcinoma 786-0 cells were used. Methyl thiazolyl tetrazo- lium （MTT） assay was used to determine the cell viability; Flow cytometry following Propidium Iodide （PI） staining was used to determine the apoptosis; Western blotting was used to detect the levels of phosphor-ERK and total ERK. The tri- al included： the control group, β-elemene group （160 μg/mL）, ERK inhibitor group （PD98059,20μmol/L）, and the combined group （PD98059＋β-elemene）. RESULTS： Under the treatment of 40, 80, 160 and 320μg/mL β-elemene for 24 h,the viability of 786-0 cells was（83.20±4.63）%,（74.29±3.50）%, （49.75±3.08）% and （16.99±4.73）%, respectively （P〈0.05）. Further study showed that the level of phosphor-ERK was down-regulated obviously under the treatment of 9-elemene. Compared with the β-elemene group, the cell viability of the combined group was reduced significantly [（52.73β6.36）% vs （39.24β3.24）%], P〈0.05. CONCLUSION：Our data indicates that the anti-tumor effect of β-elemene on RCC cells is associated with inhibition of ERK signaling pathway.

关 键 词：肾细胞癌 ERK Β-榄香烯 凋亡 

分 类 号：R737.11[医药卫生—肿瘤]