血清骨桥蛋白检测对肝癌诊断意义初步分析  

Detected clinical significance of plasma osteopontin in patients with hepatocellular carcinorna

作　　者：许英艺[1,2,3] 万幼峰[2,3] 张雅雅[2,3] 陈玉强[1,2,3] 

机构地区：[1]福建医科大学福总临床医学院,福建福州350025 [2]厦门大学附属成功医院 [3]解放军第174医院肿瘤科,福建厦门361003

出　　处：《中华肿瘤防治杂志》2012年第5期364-367,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：2009南京军区医学科技创新项目(09MB130)

摘　　要：目的:探索血清骨桥蛋白(OPN)作为肝细胞癌(HCC)生物标志的应用价值。方法:应用ELISA法检测70例HCC患者、65例慢性肝脏疾病(CLD)患者及65名健康人血清OPN、甲胎蛋白(Alpha-fetoprotein,AFP)浓度。结果:HCC组血清OPN浓度(中位数975ng/mL,范围131～5 920ng/mL)明显高于CLD组(352ng/mL,18～1 875ng/mL,P<0.001)或健康人组(103ng/mL,8～984ng/mL,P<0.001)。在HCC组,血清OPN浓度随着Child-Pugh分级(F=8.053,P=0.001)及肿瘤分期(P=0.01)的升高而显著升高,且血清OPN浓度与肿瘤包膜的完整性明显相关(P=0.001)。OPN诊断HCC的敏感度、特异度及临界值分别为87.1%、75.4%和642.5ng/mL。OPN曲线下面积(0.895±0.028)比AFP(0.817±0.04)大(P<0.01),这提示OPN有优越的诊断效能。结论:血浆中OPN浓度可以作为诊断HCC潜在的参考分子指标之一。OBJECTIVE： To investigate the usefulness of plasma OPN level as a biomarker for hepatocellular carci- noma （HCC）. METHODS： Using a standardized ELISA kit, plasma levels of osteopontin（OPN） and Alpha-fetoprotein （AFP） were measured for 70 HCC patients, 65 patients with chronic liver diseases and 65 healthy control individuals. RESULTS： Significantly higher plasma OPN levels （median 975 ng/mL, range 131-- 5 920 ng/mL） were found in the HCC patients than those in patients with chronic liver diseases （352 ng/mL, 18--1 875 ng/mL, P〈0. 001） or healthy control （103 ng/mL, range 8--984 ng/mL, P%0. 001）. Within the HCC patient group, plasma OPN level increased significantly with advancing degree of Child-Pugh class （F=8. 053,P=0. 001） and of tumor stage（P=0.01）. And the plasma OPN level was significantly correlated with capsular infiltration（P=0. 001）. Diagnostic sensitivity and specificity of OPN for HCC was 87.1% and 75.40/00, respectively （cut-off value.. 617.6 ng/mL）. OPN had a greater area under curve value （0. 895±0. 028） than AFP （0. 817±0.04, P〈0.01）, suggesting superior diagnostic accuracy of OPN. CONCLUSION：Our results suggest that plasma OPN level may be a potential diagnostic marker for HCC.

关 键 词：肝细胞癌 骨桥蛋白 酶联免疫吸附实验 

分 类 号：R735.7[医药卫生—肿瘤]