中间普氏菌特异性卵黄抗体治疗大鼠牙龈炎的研究  

Study on the Effect of Specific Egg Yolk Immunoglobulin(IgY) Against Prevotella Intermedia to Gingivitis in a Rat Model

作　　者：刘赟[1] 王丹 甄宇红[3] 龙亚一[3] 侯媛媛[3] 丁超[3] 廖瀚[3] 金月[1] 

机构地区：[1]辽宁医学院研究生院,辽宁锦州121001 [2]大连市口腔医院 [3]大连医科大学

出　　处：《口腔医学研究》2012年第5期413-416,共4页Journal of Oral Science Research

摘　　要：目的:通过动物实验考察中间普氏菌特异性卵黄抗体(IgY)对牙龈炎的治疗作用。方法:将50只SD雌性大鼠随机分为5组:空白对照组(N组)、阴性对照组(P1组):造模、阳性对照组(P2组):造模+替硝唑溶液、IgY溶液治疗组(P3组):造模+中间普氏菌特异性IgY溶液、蛋黄粉治疗组(P4组):造模+含中间普氏菌特异性IgY的蛋黄粉。对造模大鼠采用丝线结扎下前牙牙颈部,植入中间普氏菌,辅以高糖潮湿饮食,30d后造成大鼠牙龈炎模型,开始用药,每日给药1次,用含药溶液冲洗或含药蛋黄粉涂抹大鼠下前牙牙周,分别于给药0d、5d、10d、15d测定大鼠牙龈指数(GI)、菌斑指数(PLI)、探诊出血(BOP)、血液白细胞计数(WBC)及体重(Weight)的变化,用药后15d对各组大鼠进行下前牙牙龈组织病理切片检查,检查局部组织炎症变化。结果:临床与血液观察指标显示治疗组(P2、P3、P4)与P1组比较,GI、PLI、BOP及WBC均有显著降低(P<0.05),体重呈增长趋势,病理组织切片显示局部组织炎症表现较P1组为轻;P3、P4组与P2组比较,GI、PLI、BOP及WBC均有显著降低(P<0.05),体重增加显著(P<0.05),病理切片显示局部组织炎症表现较P2组为轻;P3组与P4组比较,GI、PLI、BOP及WBC均有显著降低(P<0.05),体重增加明显高于P4组(P<0.05)。结论:中间普氏菌特异性IgY两种制剂对牙龈炎均有治疗作用,溶液的治疗作用优于蛋黄粉。Objective： To estimate the effect of specific IgY against Prevotella intermedia（Pi） to gingivitis in rat. Methods： Fifty SD female rats were divided into five groups randomly： blank control group （N group） ;negative con- trol group P1 group） ：modeled; positive control group （P2 group） ：modeled and treated with tinidazole; modeled and flushed by Pi specific IgY solution （P3 group） ;modeled and smeared by yolk powder containing Pi specific IgY （P4 group）. The rats in P1 ,P2 ,P3 ,P4 group were ligatured at neck of inferior teeth, followed by infecting with Pi and fed with high sugar diets. The rats with gingivitis received treatments （flushed or smeared by different drugs） once after Pi infection. Gingival index （GI）, plaque index （PI）, bleeding on probing （BOP）, white blood cell count （WBC） and weight of the rats were measured on 0d, 5d, 10d and 15d during treatment. Tissue slices of gum from inferior teeth were prepared to ohserve local tissue inflammation on 15d. Results： Compared with P1 group, GI, PI, BOP and WBC of rats in P2 ,P3 ,P4 groups decreased significantly（P〈0.05） while weight increased significantly （P〈0.05）, and the sections showed lighter local inflammation. Compared with P2 group, rats in P3 and P4 groups showed much lower GI, PI, BOP and WBC （P〈0.05）, much higher weight （P〈0.05）, and much lighter local in- flammation. P3 group showed better efficacy than P4 group（P〈0.05）. Conclusion： Both forms of specific IgY are effective on treatment of rat gingivitis caused by Pi, and IgY solution is better than yolk powder.

关 键 词：卵黄抗体 中间普氏菌牙龈炎大鼠模型 

分 类 号：R781.41[医药卫生—口腔医学]