不同硒水平对氟中毒大鼠牙胚发育的影响  

The Effect of Different Selenium Level on Dental Germ Development of Rat with Fluorosis

作　　者：陈黎明 王颖莉 杨威 田茂能 

机构地区：[1]贵阳市口腔医院口腔内科,贵州贵阳550002

出　　处：《口腔医学研究》2012年第5期417-419,422,共4页Journal of Oral Science Research

基　　金：贵阳市社会发展攻关计划【2009】筑科农合同字第3-039号

摘　　要：目的:研究不同硒水平对氟中毒大鼠牙胚发育的影响。方法:雄性SD大鼠60只,随机分为6组。对照组饮蒸馏水饲普通饲料,实验组饮含氟(F-)45mg/L的蒸馏水,氟组饲普通饲料,氟硒1组饲含硒1.37mg/kg的饲料,氟硒2组饲含硒1.6mg/kg的饲料,氟硒3组饲含硒2.3mg/kg的饲料,氟硒四组饲含硒4mg/kg的饲料,饲养8周后断头处死大鼠,运用免疫组织化学检测技术检测smad3、shh在分泌期成釉细胞中的表达水平。结果:免疫组化结果为对照组大鼠成釉细胞中smad3、shh为阳性表达,氟组大鼠成釉细胞中smad3、shh表达比对照组明显减弱;氟硒组smad3、shh表达强于氟组,其中氟硒3组最好。结论:硒拮抗氟中毒作用的机制可能与调控牙胚发育作用的信号转导因子Smad3和Shh的表达有一定的关联,且2.3mg/kg硒效果最好。Objective： To study the effect of different selenium level on dental germ development of rat with fluoro- sis. Methods： Sixty male SD rats are divided randomly into 6 groups. The control group is fed with normal diet and distilled water. The experimental groups are fed with distilled water eonatining 45m/L fluoride （F^-）. The fluoride group is fed with normal diet. The frist fluorine and selenium group is fed with diet containing 1.37mg/kg of seleni- um. The second fluorine and selenium group is fed with diet containing 1.6mg/kg of selenium. The third fluorine and selenium group is fed with diet containing 2.3mg/kg of selenium. The fourth fluorine and selenium group is fed with diet conatining 4mg/kg of selenium. At the end of the experiment, the rats were decapitated. Immunohisto- chemistry is used to detect the expression of smad3, shh in secretory ameloblasts. Results： The expression of ameloblasts smad3 and shh is positive in the control group and, the expression in the fluoride group was significantly reduced, compared with the control group. The Fluorine and selenium groups＇ smad3 and shh expression was stron- ger than that of the fluoride groups, especially that the third group is best. Conclusion： The mechanism of the an- tagonize effect of selenium with fluorosis may be related to the expression of the signal transduction factor smad3, shh which control the dental germ development and 2.3mg/kg selenium is best.

关 键 词：氟中毒氟化钠亚硒酸钠smad3 SHH 

分 类 号：R780.2[医药卫生—口腔医学]